Document Detail


Type I and type II interferons upregulate functional type I interleukin-1 receptor in a human fibroblast cell line TIG-1.
MedLine Citation:
PMID:  8746788     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The regulation of type I interleukin-1 receptor (IL-1R) expression by type I, interferon (IFN)-alpha A/D, and type II IFN, IFN-gamma, in a human fibroblast cell line TIG-1 was investigated. After 2 h stimulation with human IFN-alpha A/D or IFN-gamma, the levels of type I IL-1R mRNA increased. We previously reported that IL-1 upregulates transcription and cell surface molecules of type I IL-1R in TIG-1 cells through induction of prostaglandin (PG) E2 and cAMP accumulation. However, indomethacin was unable to inhibit the effect of IFNs, indicating that IFNs augment IL-1R expression through a pathway distinct from that of IL-1. The augmentation was also observed in other fibroblast cell lines. Nuclear run-on assays and studies of the stability of mRNA suggested that the increase in IL-1R mRNA was a result of the enhanced transcription of IL-1R gene. Binding studies using 125I-IL-1 alpha revealed that the number of cell surface IL-1R increased with no change in binding affinity by treatment with these IFNs. Pretreatment of the cells with IFNs enhanced IL-1-induced IL-6 production, indicating that IFNs upregulate functional IL-1R. IL-1 and IFNs are produced by the same cell types, as well as by the adjacent different cell types, and are concomitantly present in lesions of immune and inflammatory reactions. These results therefore suggest that IFNs exhibit synergistic effects with IL-1 through upregulation of IL-1R. Augmented production of IL-6 may also contribute to the reactions.
Authors:
T Takii; N Niki; D Yang; H Kimura; A Ito; H Hayashi; K Onozaki
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research     Volume:  15     ISSN:  1079-9907     ISO Abbreviation:  J. Interferon Cytokine Res.     Publication Date:  1995 Dec 
Date Detail:
Created Date:  1997-02-12     Completed Date:  1997-02-12     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9507088     Medline TA:  J Interferon Cytokine Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1065-73     Citation Subset:  IM    
Affiliation:
Department of Hygienic Chemistry, Nagoya City University, Japan.
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MeSH Terms
Descriptor/Qualifier:
Cell Line
Cell Membrane / drug effects
Cycloheximide / pharmacology
Fibroblasts / drug effects,  metabolism
Humans
Interferon Type I / pharmacology*
Interferon-gamma / pharmacology*
RNA, Messenger / biosynthesis*
Receptors, Interleukin-1 / drug effects*,  genetics
Transcription, Genetic / drug effects
Up-Regulation / drug effects
Chemical
Reg. No./Substance:
0/Interferon Type I; 0/RNA, Messenger; 0/Receptors, Interleukin-1; 66-81-9/Cycloheximide; 82115-62-6/Interferon-gamma

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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