Document Detail

Type I collagen degradation by invasive oral squamous cell carcinoma.
MedLine Citation:
PMID:  10899676     Owner:  NLM     Status:  MEDLINE    
Expression of extracellular matrix-degrading proteases is required for tumor cell invasion. In the present study we examined the production of type I collagen-degrading matrix metalloproteinases (MMPs) in the invasive oral squamous cell carcinoma-derived cell line HSC-3. In the absence of serum or exogenous growth factors, HSC-3 cells displayed no collagen degradation activity. Addition of serum slightly increased collagen proteolysis. However, addition of epidermal growth factor (EGF) resulted in nearly complete degradation of the collagen matrix. Zymography showed that MMP-2 and -9 are secreted by HSC-3 cells. EGF stimulated secretion of an additional gelatinase with a molecular weight similar to that of MMP-1. Immunoblotting of conditioned medium confirmed that EGF and, to a lesser degree type I collagen, increased production of MMP-1. Finally, in situ hybridization revealed intense expression of MMP-1 in oral squamous cell carcinoma specimens. Together, these results indicate that MMP-1 is expressed, induced by EGF, and required for type I collagen degradation in oral squamous cell carcinoma.
B L Ziober; M A Turner; J M Palefsky; M J Banda; R H Kramer
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Oral oncology     Volume:  36     ISSN:  1368-8375     ISO Abbreviation:  Oral Oncol.     Publication Date:  2000 Jul 
Date Detail:
Created Date:  2000-12-07     Completed Date:  2000-12-07     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9709118     Medline TA:  Oral Oncol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  365-72     Citation Subset:  IM    
Department of Stomatology, University of California San Francisco, Box 0512, 521 Parnassus Avenue, San Francisco, CA 94143, USA.
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MeSH Terms
Blotting, Western
Carcinoma, Squamous Cell / enzymology*,  pathology
Epidermal Growth Factor / metabolism
In Situ Hybridization
Matrix Metalloproteinase 1 / metabolism*
Mouth Neoplasms / enzymology*,  pathology
Neoplasm Invasiveness / pathology
Neoplasm Proteins / metabolism*
Grant Support
Reg. No./Substance:
0/Neoplasm Proteins; 62229-50-9/Epidermal Growth Factor; 9007-34-5/Collagen; EC Metalloproteinase 1

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