Document Detail


Type and cellular location of reactive oxygen species determine activation or suppression of programmed cell death in Arabidopsis suspension cultures.
MedLine Citation:
PMID:  20383058     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
Plant programmed cell death (PCD) is a cell-controlled process that plays an essential role in development and stress responses. Apoptotic-like PCD (AL-PCD) results in a characteristic cell corpse containing a condensed cytoplasm. We recently showed that chloroplast-produced reactive oxygen species (ROS) can play a role in regulating AL-PCD. Here we show that ROS may play a variety of roles in AL-PCD regulation, depending on type and localisation of the ROS activity. Treatment of Arabidopsis thaliana cells with the antioxidants ascorbate and glutathione, which are not specific in the forms of ROS that they scavenge, resulted in increased heat stress-induced AL-PCD. However, treatment with catalase, which specifically scavenges hydrogen peroxide (H(2)O(2)) only, temporally promoted cell survival and suppressed AL-PCD after the heat treatment. These results suggest that H(2)O(2) functions as an important mobile signal that positively regulates AL-PCD in plants and that other ROS forms may play different roles in AL-PCD regulation, perhaps by acting as positive or negative regulators of components of AL-PCD signaling pathways.
Authors:
Siamsa M Doyle; Paul F McCabe
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Publication Detail:
Type:  Comment; Journal Article     Date:  2010-04-02
Journal Detail:
Title:  Plant signaling & behavior     Volume:  5     ISSN:  1559-2324     ISO Abbreviation:  Plant Signal Behav     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-10-15     Completed Date:  2011-01-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101291431     Medline TA:  Plant Signal Behav     Country:  United States    
Other Details:
Languages:  eng     Pagination:  467-8     Citation Subset:  -    
Affiliation:
School of Biology and Environmental Science, University College Dublin, Dublin, Ireland.
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Comment On:
J Exp Bot. 2010;61(2):473-82   [PMID:  19933317 ]

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