Document Detail


Type analysis of the oligosaccharide chains on microheterogeneous components of bovine pancreatic DNAase by the lectin-nitrocellulose sheet method.
MedLine Citation:
PMID:  2920025     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The oligosaccharide chains of microheterogeneous bovine pancreatic DNAases were characterized by the lectin-nitrocellulose sheet method. The active fractions of the DNAases from column chromatography showed four major and several minor spots on a two-dimensional polyacrylamide gel. They were transferred on to nitrocellulose sheets and treated with glycosidases (neuraminidase, endo-beta-N-acetyl glucosaminidase H or F, or peptide N-glycosidase F) and treated with peroxidase-coupled lectins (concanavalin A, Ricinus communis agglutinin or wheat-germ agglutinin). From the results, the most probable oligosaccharide types were proposed to be as follows: the four major spots contained components which had high-mannose type or hybrid-type oligosaccharides, such as those susceptible to endo-beta-N-acetylglucosaminidase H. In addition, spot 1 contained a complex-type biantennary oligosaccharide without sialic acid and spot 3 contained a tri- or tetra-antennary complex-type oligosaccharide with sialic acid. The component corresponding to spot 2 had a hybrid-type oligosaccharide chain with a 'bisecting' acetylglucosamine, linked 1-4 to the beta-mannose residue of the trimannosyl core, and the component corresponding to spot 4 had a high-mannose-type oligosaccharide chain.
Authors:
S Kijimoto-Ochiai; Y U Katagiri; T Hatae; H Okuyama
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Biochemical journal     Volume:  257     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1989 Jan 
Date Detail:
Created Date:  1989-04-06     Completed Date:  1989-04-06     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  43-9     Citation Subset:  IM    
Affiliation:
Institute of Immunological Science, Hokkaido University, Sapporo, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cattle
Collodion
Deoxyribonucleases / isolation & purification*
Electrophoresis, Gel, Two-Dimensional
Glycoside Hydrolases
Lectins
Methods
Oligosaccharides / isolation & purification*
Pancreas / enzymology*
Sialic Acids / isolation & purification
Chemical
Reg. No./Substance:
0/Lectins; 0/Oligosaccharides; 0/Sialic Acids; 9004-70-0/Collodion; EC 3.1.-/Deoxyribonucleases; EC 3.2.1.-/Glycoside Hydrolases
Comments/Corrections

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