| Type analysis of the oligosaccharide chains on microheterogeneous components of bovine pancreatic DNAase by the lectin-nitrocellulose sheet method. | |
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MedLine Citation:
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PMID: 2920025 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The oligosaccharide chains of microheterogeneous bovine pancreatic DNAases were characterized by the lectin-nitrocellulose sheet method. The active fractions of the DNAases from column chromatography showed four major and several minor spots on a two-dimensional polyacrylamide gel. They were transferred on to nitrocellulose sheets and treated with glycosidases (neuraminidase, endo-beta-N-acetyl glucosaminidase H or F, or peptide N-glycosidase F) and treated with peroxidase-coupled lectins (concanavalin A, Ricinus communis agglutinin or wheat-germ agglutinin). From the results, the most probable oligosaccharide types were proposed to be as follows: the four major spots contained components which had high-mannose type or hybrid-type oligosaccharides, such as those susceptible to endo-beta-N-acetylglucosaminidase H. In addition, spot 1 contained a complex-type biantennary oligosaccharide without sialic acid and spot 3 contained a tri- or tetra-antennary complex-type oligosaccharide with sialic acid. The component corresponding to spot 2 had a hybrid-type oligosaccharide chain with a 'bisecting' acetylglucosamine, linked 1-4 to the beta-mannose residue of the trimannosyl core, and the component corresponding to spot 4 had a high-mannose-type oligosaccharide chain. |
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Authors:
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S Kijimoto-Ochiai; Y U Katagiri; T Hatae; H Okuyama |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Biochemical journal Volume: 257 ISSN: 0264-6021 ISO Abbreviation: Biochem. J. Publication Date: 1989 Jan |
Date Detail:
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Created Date: 1989-04-06 Completed Date: 1989-04-06 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2984726R Medline TA: Biochem J Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 43-9 Citation Subset: IM |
Affiliation:
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Institute of Immunological Science, Hokkaido University, Sapporo, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cattle Collodion Deoxyribonucleases / isolation & purification* Electrophoresis, Gel, Two-Dimensional Glycoside Hydrolases Lectins Methods Oligosaccharides / isolation & purification* Pancreas / enzymology* Sialic Acids / isolation & purification |
| Chemical | |
Reg. No./Substance:
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0/Lectins; 0/Oligosaccharides; 0/Sialic Acids; 9004-70-0/Collodion; EC 3.1.-/Deoxyribonucleases; EC 3.2.1.-/Glycoside Hydrolases |
| Comments/Corrections | |
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