Document Detail

Type IV collagen contains two distinct chains in separate molecules.
MedLine Citation:
PMID:  7346219     Owner:  NLM     Status:  MEDLINE    
Type IV collagen from the EHS sarcoma, a basement membrane producing tumor, was characterized. Acid extracts of tumor grown in lathyritic animals contain proteins with two predominant collagen chains of apparent molecular weights of 160,000 and 140,000 daltons, designated alpha 1(IV) and alpha 2(IV). In addition, dimers and larger polymers formed by covalent crosslinking of the alpha 1(IV) and alpha 2 (IV) chains are also present. These two chains were found to be distinct from one another in amino acid composition and in the peptide patterns obtained following treatment with cyanogen bromide and S. aureus V8 protease. Precipitation of native collagen from acid solutions by the stepwise addition of NaCl yielded mixtures of the two chains with varying ratios suggesting that the two chains reside in different macromolecules.
P G Robey; G R Martin
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Collagen and related research     Volume:  1     ISSN:  0174-173X     ISO Abbreviation:  Coll. Relat. Res.     Publication Date:  1981  
Date Detail:
Created Date:  1982-10-21     Completed Date:  1982-10-21     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8102998     Medline TA:  Coll Relat Res     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  27-38     Citation Subset:  IM    
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MeSH Terms
Basement Membrane / analysis
Chemical Phenomena
Collagen / isolation & purification*
Mice, Inbred C57BL
Neoplasm Proteins / isolation & purification*
Sarcoma, Experimental / metabolism*
Reg. No./Substance:
0/Neoplasm Proteins; 9007-34-5/Collagen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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