| Type IV collagen contains two distinct chains in separate molecules. | |
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MedLine Citation:
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PMID: 7346219 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Type IV collagen from the EHS sarcoma, a basement membrane producing tumor, was characterized. Acid extracts of tumor grown in lathyritic animals contain proteins with two predominant collagen chains of apparent molecular weights of 160,000 and 140,000 daltons, designated alpha 1(IV) and alpha 2(IV). In addition, dimers and larger polymers formed by covalent crosslinking of the alpha 1(IV) and alpha 2 (IV) chains are also present. These two chains were found to be distinct from one another in amino acid composition and in the peptide patterns obtained following treatment with cyanogen bromide and S. aureus V8 protease. Precipitation of native collagen from acid solutions by the stepwise addition of NaCl yielded mixtures of the two chains with varying ratios suggesting that the two chains reside in different macromolecules. |
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Authors:
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P G Robey; G R Martin |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Collagen and related research Volume: 1 ISSN: 0174-173X ISO Abbreviation: Coll. Relat. Res. Publication Date: 1981 |
Date Detail:
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Created Date: 1982-10-21 Completed Date: 1982-10-21 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 8102998 Medline TA: Coll Relat Res Country: GERMANY, WEST |
Other Details:
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Languages: eng Pagination: 27-38 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Basement Membrane / analysis Chemical Phenomena Chemistry Collagen / isolation & purification* Female Mice Mice, Inbred C57BL Neoplasm Proteins / isolation & purification* Sarcoma, Experimental / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Neoplasm Proteins; 9007-34-5/Collagen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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