| Type II pneumocyte changes during hyperoxic lung injury and recovery. | |
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MedLine Citation:
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PMID: 3215867 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Adult rabbits exposed to 100% O2 for 64 h and then returned to room air for up to 200 h, develop a lung injury characterized by decreased levels of alveolar surfactant followed by a rebound recovery. In the present study we isolated alveolar type II cells from rabbits at various times during hyperoxic exposure and recovery and measured rates of phosphatidylcholine (PC) synthesis, cellular lipid content, and the specific activity of glycerol 3-phosphate (G-3-P) acyltransferase, an enzyme that catalyzes one of the early reactions in phosphoglyceride biosynthesis. These biochemical parameters were compared with measurements of cell size and cell cycle phase by laser flow cytometry. Results showed that alterations in alveolar phospholipid levels in vivo correlated consistently with cellular lipid metabolic changes measured in isolated type II pneumocytes. In particular, alveolar pneumocytes isolated from lungs of rabbits exposed to 100% O2 for 64 h exhibited a 60% decrease in PC synthesis, cell lipid content, and G-3-P acyltransferase activity. All variables then followed a pattern of recovery to normal and ultimately supranormal levels beginning at approximately 3 days postexposure, at which point there was also a measured increase in the number of type II cells in S phase. These findings suggest that O2-induced changes in type II cell surfactant biosynthesis may account, at least in part, for observed changes in lung phospholipid levels in vivo. |
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Authors:
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B A Holm; S Matalon; J N Finkelstein; R H Notter |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of applied physiology (Bethesda, Md. : 1985) Volume: 65 ISSN: 8750-7587 ISO Abbreviation: J. Appl. Physiol. Publication Date: 1988 Dec |
Date Detail:
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Created Date: 1989-03-09 Completed Date: 1989-03-09 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 8502536 Medline TA: J Appl Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 2672-8 Citation Subset: IM |
Affiliation:
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Department of Biophysics, University of Rochester, New York 14642. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Cycle Cell Division DNA / biosynthesis Glycerol-3-Phosphate O-Acyltransferase / metabolism Lung / metabolism, pathology Lung Injury* Oxygen* Phospholipids / biosynthesis Pulmonary Alveoli / metabolism, pathology* Pulmonary Surfactants / biosynthesis Rabbits |
| Grant Support | |
ID/Acronym/Agency:
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HL-07216/HL/NHLBI NIH HHS; HL-31197/HL/NHLBI NIH HHS; HL-37388/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Phospholipids; 0/Pulmonary Surfactants; 7782-44-7/Oxygen; 9007-49-2/DNA; EC 2.3.1.15/Glycerol-3-Phosphate O-Acyltransferase |
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