| Type 6 secretion dynamics within and between bacterial cells. | |
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MedLine Citation:
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PMID: 22767897 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The bacterial type 6 secretion system (T6SS) functions as a virulence factor capable of attacking both eukaryotic and prokaryotic target cells by a process that involves protein transport through a contractile bacteriophage tail-like structure. The T6SS apparatus is composed, in part, of an exterior sheath wrapped around an interior tube. Here, we report that in living cells the cytoplasmic adenosine triphosphatase called ClpV specifically recognizes the contracted T6SS sheath structure, causing its disassembly within seconds. ClpV imaging allowed spatial and temporal documentation of cell-cell interactions (termed T6SS dueling) that likely mark the location of repeated T6SS-mediated protein translocation events between bacterial cells. |
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Authors:
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M Basler; J J Mekalanos |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2012-07-05 |
Journal Detail:
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Title: Science (New York, N.Y.) Volume: 337 ISSN: 1095-9203 ISO Abbreviation: Science Publication Date: 2012 Aug |
Date Detail:
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Created Date: 2012-08-20 Completed Date: 2012-08-28 Revised Date: 2013-04-12 |
Medline Journal Info:
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Nlm Unique ID: 0404511 Medline TA: Science Country: United States |
Other Details:
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Languages: eng Pagination: 815 Citation Subset: IM |
Affiliation:
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Department of Microbiology and Immunobiology, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alanine
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genetics,
metabolism Amino Acid Substitution Bacterial Proteins / genetics, metabolism* Bacterial Secretion Systems* Green Fluorescent Proteins / genetics, metabolism Microscopy, Fluorescence / methods Molecular Imaging / methods Mutation Protein Transport Pseudomonas aeruginosa / metabolism, physiology* Recombinant Fusion Proteins / genetics, metabolism Tyrosine / genetics, metabolism Vibrio cholerae / metabolism, physiology* |
| Grant Support | |
ID/Acronym/Agency:
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AI-018045/AI/NIAID NIH HHS; AI-26289/AI/NIAID NIH HHS; R01 AI018045/AI/NIAID NIH HHS; R01 AI026289/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/Recombinant Fusion Proteins; 147336-22-9/Green Fluorescent Proteins; 55520-40-6/Tyrosine; 56-41-7/Alanine |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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