Document Detail


Type 6 secretion dynamics within and between bacterial cells.
MedLine Citation:
PMID:  22767897     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The bacterial type 6 secretion system (T6SS) functions as a virulence factor capable of attacking both eukaryotic and prokaryotic target cells by a process that involves protein transport through a contractile bacteriophage tail-like structure. The T6SS apparatus is composed, in part, of an exterior sheath wrapped around an interior tube. Here, we report that in living cells the cytoplasmic adenosine triphosphatase called ClpV specifically recognizes the contracted T6SS sheath structure, causing its disassembly within seconds. ClpV imaging allowed spatial and temporal documentation of cell-cell interactions (termed T6SS dueling) that likely mark the location of repeated T6SS-mediated protein translocation events between bacterial cells.
Authors:
M Basler; J J Mekalanos
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-07-05
Journal Detail:
Title:  Science (New York, N.Y.)     Volume:  337     ISSN:  1095-9203     ISO Abbreviation:  Science     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-08-20     Completed Date:  2012-08-28     Revised Date:  2013-07-12    
Medline Journal Info:
Nlm Unique ID:  0404511     Medline TA:  Science     Country:  United States    
Other Details:
Languages:  eng     Pagination:  815     Citation Subset:  IM    
Affiliation:
Department of Microbiology and Immunobiology, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA.
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MeSH Terms
Descriptor/Qualifier:
Alanine / genetics,  metabolism
Amino Acid Substitution
Bacterial Proteins / genetics,  metabolism*
Bacterial Secretion Systems*
Green Fluorescent Proteins / genetics,  metabolism
Microscopy, Fluorescence / methods
Molecular Imaging / methods
Mutation
Protein Transport
Pseudomonas aeruginosa / metabolism,  physiology*
Recombinant Fusion Proteins / genetics,  metabolism
Tyrosine / genetics,  metabolism
Vibrio cholerae / metabolism,  physiology*
Grant Support
ID/Acronym/Agency:
AI-018045/AI/NIAID NIH HHS; AI-26289/AI/NIAID NIH HHS; R01 AI018045/AI/NIAID NIH HHS; R01 AI026289/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Recombinant Fusion Proteins; 147336-22-9/Green Fluorescent Proteins; 55520-40-6/Tyrosine; 56-41-7/Alanine
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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