Document Detail


TyeA, a protein involved in control of Yop release and in translocation of Yersinia Yop effectors.
MedLine Citation:
PMID:  9524114     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Extracellular Yersinia spp. disarm the immune system by injecting the effector Yersinia outer proteins (Yops) into the target cell. Yop secretion is triggered by contact with eukaryotic cells or by Ca2+ chelation. Two proteins, YopN and LcrG, are known to be involved in Yop-secretion control. Here we describe TyeA, a third protein involved in the control of Yop release. Like YopN, TyeA is localized at the bacterial surface. A tyeA knock-out mutant secreted Yops in the presence of Ca2+ and in the absence of eukaryotic cells. Unlike a yopN null mutant, the tyeA mutant was defective for translocation of YopE and YopH, but not YopM, YopO and YopP, into eukaryotic cells. This is the first observation suggesting that Yop effectors can be divided into two sets for delivery into eukaryotic cells. TyeA was found to interact with the translocator YopD and with residues 242-293 of YopN. In contrast with a yopN null mutant, a yopNDelta248-272 mutant was also unable to translocate YopE and YopH. Our results suggest that TyeA forms part of the translocation-control apparatus together with YopD and YopN, and that the interaction of these proteins is required for selective translocation of Yops inside eukaryotic cells.
Authors:
M Iriarte; M P Sory; A Boland; A P Boyd; S D Mills; I Lambermont; G R Cornelis
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The EMBO journal     Volume:  17     ISSN:  0261-4189     ISO Abbreviation:  EMBO J.     Publication Date:  1998 Apr 
Date Detail:
Created Date:  1998-06-01     Completed Date:  1998-06-01     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  8208664     Medline TA:  EMBO J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1907-18     Citation Subset:  IM    
Affiliation:
Microbial Pathogenesis Unit, Christian de Duve Institute of Cellular Pathology and Faculté de Médecine, Université Catholique de Louvain, Avenue Hippocrate 74, UCL 74.49, B-1200 Brussels, Belgium.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AF102990
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MeSH Terms
Descriptor/Qualifier:
Apoptosis
Bacterial Outer Membrane Proteins / analysis,  genetics,  metabolism*,  physiology*
Bacterial Proteins / chemistry,  genetics,  metabolism*
Biological Transport
Calcium / metabolism
Carrier Proteins / analysis,  genetics,  metabolism,  physiology*
Cell Line
Cell Membrane / chemistry
Macrophages / cytology,  microbiology
Membrane Proteins*
Molecular Sequence Data
Open Reading Frames / genetics
Plasmids / genetics
Recombinant Fusion Proteins
Virulence
Yersinia enterocolitica / chemistry,  genetics,  metabolism,  pathogenicity*
Chemical
Reg. No./Substance:
0/Bacterial Outer Membrane Proteins; 0/Bacterial Proteins; 0/Carrier Proteins; 0/Membrane Proteins; 0/Recombinant Fusion Proteins; 0/TyeA protein, Yersinia; 138341-76-1/YopN protein, Yersinia; 7440-70-2/Calcium
Comments/Corrections

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