| Two unusual glycerophospholipids from a filamentous fungus, Absidia corymbifera. | |
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MedLine Citation:
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PMID: 11325609 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The chloroform-methanol extractable lipids of the soil filamentous fungus Absidia corymbifera VKMF-965 account for about 20% by weight of dry cells and are composed of low-polarity constituents (about 75% of the total lipids), such as triacylglycerols (mainly), diacylglycerols, sterols and free fatty acids, as well as of glycolipids (about 3%) and phospholipids. The last consist largely of components common to the fungal lipids, namely, phosphatidylethanolamine (38% of the total phospholipids), phosphatidyl-myo-inositol (16%), diphosphatidylglycerol (12%), phosphatidylcholine (7%), phosphatidic acid (6%) and phosphatidylglycerol (3%), and two unusual phospholipids, PL1 (6%) and PL2 (9%). Based on the infrared (IR), (1)H-nuclear magnetic resonance (NMR), (13)C-NMR and mass spectra along with the results of degradation experiment, these two phospholipids have been established to be 1,2-diacyl-sn-glycero-3-phospho(N-acetylethanolamine), or N-acetyl phosphatidylethanolamine, and 1,2-diacyl-sn-glycero-3-phospho(N-ethoxycarbonyl-ethanolamine), respectively. These structures have been confirmed by preparing similar phospholipids from the phosphatidylethanolamine isolated from the same fungus and correlating their chromatographic behaviour, IR and (1)H-NMR spectra with those of PL1 and PL2. So far N-acetyl phosphatidylethanolamine has been detected only in cattle and human brains and a human placenta but its structure was not rigorously proved. PL2 is a novel lipid; to our knowledge no natural phospholipid with an urethane group has yet been found. The main fatty acids of both the phospholipids are n-hexadecanoic, octadecanoic and octadecadienoic ones; PL2 contains in addition a considerable amount of octadecatrienoic acid with its greater portion located at the sn-1 position. |
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Authors:
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S G Batrakov; I V Konova; V I Sheichenko; S E Esipov; L A Galanina |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 1531 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 2001 Apr |
Date Detail:
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Created Date: 2001-04-30 Completed Date: 2001-06-07 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: Netherlands |
Other Details:
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Languages: eng Pagination: 169-77 Citation Subset: IM |
Affiliation:
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Research Centre Hydrobios of Ministry of Health, Moscow, Russia. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Absidia
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chemistry*,
genetics Fatty Acids / analysis Glycerophospholipids / chemistry*, isolation & purification Magnetic Resonance Spectroscopy Molecular Structure Phosphatidylethanolamines / chemistry Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Spectrophotometry, Infrared |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 0/Glycerophospholipids; 0/Phosphatidylethanolamines |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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