| Two types of human pancreatic gamma-glutamyl transpeptidase. | |
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MedLine Citation:
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PMID: 2859133 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Two different types of gamma-glutamyl transpeptidase (gamma-GTP) were extracted from human pancreas by protease treatments such as bromelain. Furthermore, human pancreatic gamma-GTP, extracted with trypsin, was separated into two different components by additional treatment with bromelain. One component displayed fast electrophoretic mobility during polyacrylamide gel electrophoresis and an apparent affinity for an anion-exchange column, while the other showed slow electrophoretic mobility and passed through the anion-exchange column with starting buffer. In addition, the percentage affinity to concanavalin A (Con A) of the former was 52.2% and of the latter only 7.2%. On heat stability, the former was more sensitive than the latter at 56 degrees C. These results indicate the existence of two types of gamma-GTP in human pancreas. |
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Authors:
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N Yamaguchi; M Sugimoto; K Kawai |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Clinica chimica acta; international journal of clinical chemistry Volume: 147 ISSN: 0009-8981 ISO Abbreviation: Clin. Chim. Acta Publication Date: 1985 Apr |
Date Detail:
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Created Date: 1985-06-18 Completed Date: 1985-06-18 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 1302422 Medline TA: Clin Chim Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 75-83 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bromelains
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pharmacology Chromatography, Affinity Chromatography, Ion Exchange Concanavalin A Electrophoresis, Polyacrylamide Gel Humans Isoenzymes / isolation & purification, metabolism* Kinetics Molecular Weight Pancreas / enzymology* Trypsin / pharmacology gamma-Glutamyltransferase / isolation & purification, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Isoenzymes; 11028-71-0/Concanavalin A; 9001-00-7/Bromelains; EC 2.3.2.2/gamma-Glutamyltransferase; EC 3.4.21.4/Trypsin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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