Document Detail

Two time-point assessment of bile acid kinetics in humans using stable isotopes.
MedLine Citation:
PMID:  20645205     Owner:  NLM     Status:  MEDLINE    
The stable isotope dilution measurement of bile acid pool sizes and turnover rates in humans has involved the collection of nine blood samples over four days. This precludes widespread application to larger population studies. This study describes a two time-point approach for blood sampling without loss of statistical power. Isotopic decay curves of cholic acid, chenodeoxycholic acid and deoxycholic acid acquired in three recent human studies were analysed. The optimal combination of two time-points was determined. Time-points around 12 and 72 h after administration allowed for the most accurate description of the decay curves and prediction of kinetic parameters. Analyses of 39 statistical comparisons of kinetic parameters based upon the two time-points and all time-points approaches exhibited only one slightly discrepant result. In conclusion, for group comparison of bile acid kinetics in humans, a two time-point blood collection approach at time-points near 12 and 72 h provides statistically reliable data.
Frans Stellaard; Vincent W Bloks; Hans G M Burgerhof; Richard A Scheltema; Elizabeth J Murphy; Hans A Romijn; Guus H M Smelt; Gemma Brufau; Folkert Kuipers
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Isotopes in environmental and health studies     Volume:  46     ISSN:  1477-2639     ISO Abbreviation:  Isotopes Environ Health Stud     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-09-16     Completed Date:  2011-01-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9602611     Medline TA:  Isotopes Environ Health Stud     Country:  England    
Other Details:
Languages:  eng     Pagination:  325-36     Citation Subset:  IM    
Department of Laboratory Medicine, University Medical Center, Groningen, The Netherlands.
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MeSH Terms
Bile Acids and Salts / blood*
Carbon Isotopes / analysis*
Chenodeoxycholic Acid / blood
Cholic Acid / blood
Deoxycholic Acid / blood
Deuterium / analysis*
Diabetes Mellitus, Type 2 / blood
Hypertriglyceridemia / blood
Isotope Labeling / methods
Obesity / blood
Radioisotope Dilution Technique
Reference Values
Time Factors
Reg. No./Substance:
0/Bile Acids and Salts; 0/Carbon Isotopes; 474-25-9/Chenodeoxycholic Acid; 7782-39-0/Deuterium; 81-25-4/Cholic Acid; 83-44-3/Deoxycholic Acid

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