| Two time-point assessment of bile acid kinetics in humans using stable isotopes. | |
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MedLine Citation:
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PMID: 20645205 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The stable isotope dilution measurement of bile acid pool sizes and turnover rates in humans has involved the collection of nine blood samples over four days. This precludes widespread application to larger population studies. This study describes a two time-point approach for blood sampling without loss of statistical power. Isotopic decay curves of cholic acid, chenodeoxycholic acid and deoxycholic acid acquired in three recent human studies were analysed. The optimal combination of two time-points was determined. Time-points around 12 and 72 h after administration allowed for the most accurate description of the decay curves and prediction of kinetic parameters. Analyses of 39 statistical comparisons of kinetic parameters based upon the two time-points and all time-points approaches exhibited only one slightly discrepant result. In conclusion, for group comparison of bile acid kinetics in humans, a two time-point blood collection approach at time-points near 12 and 72 h provides statistically reliable data. |
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Authors:
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Frans Stellaard; Vincent W Bloks; Hans G M Burgerhof; Richard A Scheltema; Elizabeth J Murphy; Hans A Romijn; Guus H M Smelt; Gemma Brufau; Folkert Kuipers |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Isotopes in environmental and health studies Volume: 46 ISSN: 1477-2639 ISO Abbreviation: Isotopes Environ Health Stud Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-09-16 Completed Date: 2011-01-10 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9602611 Medline TA: Isotopes Environ Health Stud Country: England |
Other Details:
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Languages: eng Pagination: 325-36 Citation Subset: IM |
Affiliation:
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Department of Laboratory Medicine, University Medical Center, Groningen, The Netherlands. f.stellaard@med.umcg.nl |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Bile Acids and Salts
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blood* Carbon Isotopes / analysis* Chenodeoxycholic Acid / blood Cholic Acid / blood Deoxycholic Acid / blood Deuterium / analysis* Diabetes Mellitus, Type 2 / blood Female Humans Hypertriglyceridemia / blood Isotope Labeling / methods Kinetics Male Obesity / blood Radioisotope Dilution Technique Reference Values Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Bile Acids and Salts; 0/Carbon Isotopes; 474-25-9/Chenodeoxycholic Acid; 7782-39-0/Deuterium; 81-25-4/Cholic Acid; 83-44-3/Deoxycholic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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