Document Detail


Two subpopulations of Listeria monocytogenes occur at subinhibitory concentrations of leucocin 4010 and nisin.
MedLine Citation:
PMID:  16461719     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In situ analyses of single Listeria monocytogenes cells at subinhibitory concentrations of leucocin 4010 and nisin revealed two subpopulations when measured by fluorescence ratio imaging microscopy (FRIM) after staining with 5(6)-carboxyfluorescein diacetate succinimidyl ester. One subpopulation consisted of cells with a dissipated pH gradient (DeltapH), and the other consisted of cells that maintained DeltapH. The proportion of cells belonging to each subpopulation was estimated, and the concentrations of bacteriocins required to dissipate DeltapH for 90% of the cell population (ED90) was predicted. ED90 increased after the addition of sodium chloride (1 to 3% [wt/vol]) to the bacteriocin solutions, while ED90 decreased by the addition of sodium nitrite (60 and 100 ppm). Other meat additives, including sodium phosphate, sodium lactate, sodium citrate, and sodium acetate slightly increased ED90. The inhibitory effect of sodium chloride on the antilisterial activity of leucocin 4010 and nisin was confirmed on the surfaces of meat sausages. This study highlights the important practical implications of applying subinhibitory concentrations of bacteriocins, which results in unaffected target cells. In situ analyses by FRIM in combination with modeling of single-cell data can be applied to ensure that sufficient concentrations of bacteriocins are used in food preservation.
Authors:
Tina Hornbaek; Per B Brockhoff; Henrik Siegumfeldt; Birgitte Bjørn Budde
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  72     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2006 Feb 
Date Detail:
Created Date:  2006-02-07     Completed Date:  2006-04-20     Revised Date:  2013-06-07    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1631-8     Citation Subset:  IM    
Affiliation:
Department of Food Science, The Royal Veterinary and Agricultural University, Frederiksberg, Denmark.
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MeSH Terms
Descriptor/Qualifier:
Bacteriocins / administration & dosage*
Cells, Immobilized
Food Microbiology
Food Preservation
Listeria monocytogenes / classification,  drug effects*
Meat / microbiology
Microscopy, Fluorescence
Models, Biological
Nisin / administration & dosage*
Chemical
Reg. No./Substance:
0/Bacteriocins; 1414-45-5/Nisin
Comments/Corrections

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