Document Detail


Two-step differentiation of mast cells from induced pluripotent stem cells.
MedLine Citation:
PMID:  23045993     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Mast cells play important roles in the pathogenesis of allergic diseases. They are generally classified into two phenotypically distinct populations: connective tissue-type mast cells (CTMCs) and mucosal-type mast cells (MMCs). The number of mast cells that can be obtained from tissues is limited, making it difficult to study the function of mast cells. Here, we report the generation and characterization of CTMC-like mast cells derived from mouse induced pluripotent stem (iPS) cells. iPS cell-derived mast cells (iPSMCs) were generated by the OP9 co-culture method or EB formation method. The number of Safranin O-positive cells, expression levels of CD81 protein and histidine decarboxylase (HDC) mRNA, and protease activities, were elevated in the iPSMCs differentiated by both methods as compared with those in bone marrow-derived mast cells (BMMCs). Electron microscopic analysis revealed that iPSMCs contained more granules than BMMCs. Degranulation was induced in iPSMCs after stimulation with cationic secretagogues or vancomycin. In addition, iPSMCs had the ability to respond to stimulation with the IgE/antigen complex in vitro and in vivo. Moreover, when iPSMCs generated on OP9 cells were co-cultured with Swiss 3T3 fibroblasts, protease activities as maturation index were more elevated, demonstrating that mature mast cells were differentiated from iPS cells. iPSMCs can be used as an in vitro model of CTMCs to investigate their functions.
Authors:
Tomoko Yamaguchi; Katsuhisa Tashiro; Satoshi Tanaka; Sumie Katayama; Waka Ishida; Ken Fukuda; Atsuki Fukushima; Ryoko Araki; Masumi Abe; Hiroyuki Mizuguchi; Kenji Kawabata
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-9
Journal Detail:
Title:  Stem cells and development     Volume:  -     ISSN:  1557-8534     ISO Abbreviation:  Stem Cells Dev.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101197107     Medline TA:  Stem Cells Dev     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
National Institute of Biomedical Innovation, Laboratory of Stem Cell Regulation, Ibaraki, Osaka, Japan; t-yamaguchi@nibio.go.jp.
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