| Two-step derivatization procedures for the ionization enhancement of anabolic steroids in LC-ESI-MS for doping control analysis. | |
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MedLine Citation:
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PMID: 22250799 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Background: Two-step derivatization procedures were developed for the enhancement of the positive ESI in LC-MS detection of anabolic androgenic steroids, a class of prohibited substances with limited ionization efficiency in atmospheric pressure interfaces. The developed procedures are based on the esterification of hydroxyl groups of anabolic steroids with picolinic acid, followed by conversion of carbonyl groups to Schiff bases by either Girard's reagent T or 2-hydrazino pyridin. Results: Ionization efficiency for the model derivatized compounds 19-norandrosterone (nandrolone main metabolite) and methasterone was higher by almost two orders of magnitude compared with the respective efficiency of the underivatized compounds. Conclusion: The obtained derivatives provided a significant improvement in the ESI sensitivity, compared with those of underivatized molecules in positive LC-ESI-ion trap-MS full-scan mode. |
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Authors:
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I Athanasiadou; Ys Angelis; E Lyris; A Vonaparti; Ns Thomaidis; Ma Koupparis; C Georgakopoulos |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Bioanalysis Volume: 4 ISSN: 1757-6199 ISO Abbreviation: Bioanalysis Publication Date: 2012 Jan |
Date Detail:
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Created Date: 2012-01-18 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101512484 Medline TA: Bioanalysis Country: England |
Other Details:
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Languages: eng Pagination: 167-75 Citation Subset: IM |
Affiliation:
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Doping Control Laboratory of Athens, Olympic Athletic Center of Athens, Kifissias 37, 15123 Maroussi, Greece. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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