| Two-step cell-death kinetics in vitro during cis-platinum, hydroxyurea and mitomycin incubation. | |
| | |
MedLine Citation:
|
PMID: 6435875 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
A human leukaemic cell line (REH) growing in suspension was incubated with cis-platinum, hydroxyurea and mitomycin C at various concentrations causing complete cell-cycle arrest. At different times the cell suspensions were harvested, diluted 1:1 with a buffer solution, stained without further treatment with a mixture of acridine orange (AO) and ethidium bromide (EB) and analysed with a biparametrical flow cytometer. Fluorescent plastic beads were introduced into the suspensions to provide an internal numerical reference for the control of cell loss. The fluorescence distributions showed three groups of cells: vital cells (V) which were only stained with AO; dead cells in which EB stained cytoplasmic components but not the nuclear DNA (D1), and dead cells which allowed EB to stain both cytoplasm and nuclear DNA (D2). The kinetics of cells entering D1 depended on drug concentration and showed equal characteristics for cis-platinum and mitomycin, but were different for hydroxyurea. The subsequent entry into D2 occurred about 15 hr later and showed no pronounced dependence on drug concentration. Parallel trypan-blue (TB) exclusion tests revealed that TB only stained D2 cells and therefore is not useful for investigating cell-death kinetics during exposure to cell-killing agents. |
| | |
Authors:
|
R M Böhmer |
Related Documents
:
|
20550705 - Dynamics and mechanisms of quantum dot nanoparticle cellular uptake. 8354125 - Detection of rare cells at a frequency of one per million by flow cytometry. 19938185 - A microwell array device with integrated microfluidic components for enhanced single-ce... 18397775 - Fluorescence labeling of a cytokine with desthiobiotin-tagged fluorescent puromycin. 23030945 - Stick-slip motion and elastic coupling in crawling cells. 16752805 - Are both embryonic migratory pathways preserved in the adult brain cerebral cortex? |
Publication Detail:
|
Type: Comparative Study; Journal Article |
Journal Detail:
|
Title: Cell and tissue kinetics Volume: 17 ISSN: 0008-8730 ISO Abbreviation: Cell Tissue Kinet Publication Date: 1984 Nov |
Date Detail:
|
Created Date: 1984-12-18 Completed Date: 1984-12-18 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0174107 Medline TA: Cell Tissue Kinet Country: ENGLAND |
Other Details:
|
Languages: eng Pagination: 593-600 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Antibiotics, Antineoplastic
/
toxicity* Cell Line Cell Survival / drug effects* Cisplatin / toxicity* Flow Cytometry Humans Hydroxyurea / toxicity* Kinetics Leukemia Mitomycin Mitomycins / toxicity* |
| Chemical | |
Reg. No./Substance:
|
0/Antibiotics, Antineoplastic; 0/Mitomycins; 127-07-1/Hydroxyurea; 15663-27-1/Cisplatin; 50-07-7/Mitomycin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Critical comments on galactosyltransferase.
Next Document: Comparative immunocytochemical study of the subcommissural organ.