Document Detail


Two specific populations of GABAergic neurons originating from the medial and the caudal ganglionic eminences aid in proper navigation of callosal axons.
MedLine Citation:
PMID:  23420573     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The corpus callosum (CC) plays a crucial role in interhemispheric communication. It has been shown that CC formation relies on the guidepost cells located in the midline region that include glutamatergic and GABAergic neurons as well as glial cells. However, the origin of these guidepost GABAergic neurons and their precise function in callosal axon pathfinding remain to be investigated. Here, we show that two distinct GABAergic neuronal subpopulations converge toward the midline prior to the arrival of callosal axons. Using in vivo and ex vivo fate mapping we show that CC GABAergic neurons originate in the caudal and medial ganglionic eminences (CGE and MGE) but not in the lateral ganglionic eminence (LGE). Time lapse imaging on organotypic slices and in vivo analyses further revealed that CC GABAergic neurons contribute to the normal navigation of callosal axons. The use of Nkx2.1 knockout (KO) mice confirmed a role of these neurons in the maintenance of proper behavior of callosal axons while growing through the CC. Indeed, using in vitro transplantation assays, we demonstrated that both MGE- and CGE-derived GABAergic neurons exert an attractive activity on callosal axons. Furthermore, by combining a sensitive RT-PCR technique with in situ hybridization, we demonstrate that CC neurons express multiple short and long range guidance cues. This study strongly suggests that MGE- and CGE-derived interneurons may guide CC axons by multiple guidance mechanisms and signaling pathways.
Authors:
Mathieu Niquille; Shilpi Minocha; Jean-Pierre Hornung; Nathalie Rufer; Delphine Valloton; Nicoletta Kessaris; Fabienne Alfonsi; Tania Vitalis; Yuchio Yanagawa; Christiane Devenoges; Alexandre Dayer; Cécile Lebrand
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't     Date:  2013-08-02
Journal Detail:
Title:  Developmental neurobiology     Volume:  73     ISSN:  1932-846X     ISO Abbreviation:  Dev Neurobiol     Publication Date:  2013 Sep 
Date Detail:
Created Date:  2013-08-14     Completed Date:  2013-12-31     Revised Date:  2014-02-20    
Medline Journal Info:
Nlm Unique ID:  101300215     Medline TA:  Dev Neurobiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  647-72     Citation Subset:  IM    
Copyright Information:
Copyright © 2013 Wiley Periodicals, Inc.
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MeSH Terms
Descriptor/Qualifier:
Animals
Axons / physiology*
Cell Movement / physiology*
Corpus Callosum / embryology*,  ultrastructure
Female
GABAergic Neurons / cytology*,  ultrastructure
Interneurons / cytology
Male
Mice
Mice, Knockout
Neurogenesis / physiology
Nuclear Proteins / genetics
Telencephalon / cytology,  embryology
Transcription Factors / genetics
Grant Support
ID/Acronym/Agency:
G0501173//Medical Research Council; //Wellcome Trust
Chemical
Reg. No./Substance:
0/Nuclear Proteins; 0/Transcription Factors; 0/thyroid nuclear factor 1

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