Document Detail


Two-site comparison of broth microdilution and semisolid agar dilution methods for susceptibility testing of Cryptococcus neoformans in three media.
MedLine Citation:
PMID:  8501245     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study evaluated the inter- and intralaboratory agreement between results of the semisolid agar dilution and broth microdilution methods of antifungal susceptibility testing of Cryptococcus neoformans. Three media were tested in two laboratories. The drugs tested were amphotericin B, flucytosine, itraconazole, fluconazole, and Schering 39304. Analysis by kappa statistics revealed good agreement between the laboratories for the two methods. The highest level of inter- and intralaboratory agreement was observed in RPMI 1640 with L-glutamine followed by Eagle's minimum essential medium and yeast nitrogen broth. The broth microdilution method appears more suitable than the semisolid agar dilution method for testing cryptococci because of its ease in performance, cost, and simplicity.
Authors:
E Anaissie; R Shawar; V Paetznick; L G Ensign; Z Witte; M LaRocco
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  31     ISSN:  0095-1137     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  1993 May 
Date Detail:
Created Date:  1993-06-29     Completed Date:  1993-06-29     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1370-2     Citation Subset:  IM    
Affiliation:
Department of Infectious Diseases, University of Texas M.D. Anderson Cancer Center, Houston 77030.
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MeSH Terms
Descriptor/Qualifier:
Agar
Antifungal Agents / pharmacology
Cryptococcosis / drug therapy
Cryptococcus neoformans / drug effects*,  isolation & purification
Culture Media
Evaluation Studies as Topic
Humans
Microbial Sensitivity Tests / methods*,  statistics & numerical data
Reproducibility of Results
Grant Support
ID/Acronym/Agency:
CA 16672/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Antifungal Agents; 0/Culture Media; 9002-18-0/Agar
Comments/Corrections

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