Document Detail


Two processes responsible for chemiluminescence development in the course of iron-mediated lipid peroxidation.
MedLine Citation:
PMID:  8726583     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The kinetics of chemiluminescence (CL) accompanying Fe(2+)-induced lipid peroxidation (LPO) in liposome suspension has been investigated. A sequence of stages was observed, namely: (1) fast CL flash (FF); (2) latent period (LP); (3) slow CL flash (SF) and (4) stationary chemiluminescence (SL). The first three stages are known to reflect the Fe(2+)-mediated LPO process. In spite of the fact that at the stage of SL Fe2+ has completely oxidized and MDA has not accumulated, CL intensity was found to increase and after 0.5-1 h reached a value that was several times higher than SF amplitude. The maximal SL level was linearly dependent on the initial Fe2+ concentration and was not dependent on liposome concentration in the suspension. The nature of the processes responsible for CL emission at the stage of SL has been investigated using free radical reaction inhibitors and measurement of CL spectra. The SL spectrum was observed in the red region (lamda > 590 nm) in contrast to the SF CL spectrum (maximum at 540nm). SL amplitude was strongly inhibited by sodium azide (40%), superoxide dismutase (SOD) (30%), desferrioxamine and EDTA (30%), whereas mannitol, ethanol, alpha-tocopherol and butylated hydroxytoluene were ineffective. The data obtained indicate that CL at the stage of SL is not directly related to LPO process, i.e. lipid free radical recombination. The mechanism of stationary CL generation is discussed.
Authors:
V S Sharov; E S Driomina; Y A Vladimirov
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of bioluminescence and chemiluminescence     Volume:  11     ISSN:  0884-3996     ISO Abbreviation:  J. Biolumin. Chemilumin.     Publication Date:    1996 Mar-Apr
Date Detail:
Created Date:  1996-10-18     Completed Date:  1996-10-18     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8612490     Medline TA:  J Biolumin Chemilumin     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  91-8     Citation Subset:  IM    
Affiliation:
Institute of Physical-Chemical Medicine, Moscow, Russia.
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MeSH Terms
Descriptor/Qualifier:
Azides
Butylated Hydroxytoluene
Chemiluminescent Measurements*
Deferoxamine
Edetic Acid
Ethanol
Iron*
Kinetics
Lipid Peroxidation*
Liposomes
Mannitol
Phospholipids / chemistry*
Sodium Azide
Superoxide Dismutase
Time Factors
Vitamin E
Chemical
Reg. No./Substance:
0/Azides; 0/Liposomes; 0/Phospholipids; 128-37-0/Butylated Hydroxytoluene; 1406-18-4/Vitamin E; 26628-22-8/Sodium Azide; 60-00-4/Edetic Acid; 64-17-5/Ethanol; 69-65-8/Mannitol; 70-51-9/Deferoxamine; 7439-89-6/Iron; EC 1.15.1.1/Superoxide Dismutase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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