Document Detail


Two populations of double minute chromosomes harbor distinct amplicons, the MYC locus at 8q24.2 and a 0.43-Mb region at 14q24.1, in the SW613-S human carcinoma cell line.
MedLine Citation:
PMID:  19372663     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
High-level amplifications observed in tumor cells are usually indicative of genes involved in oncogenesis. We report here a high resolution characterization of a new amplified region in the SW613-S carcinoma cell line. This cell line contains tumorigenic cells displaying high-level MYC amplification in the form of double minutes (DM(+) cells) and non tumorigenic cells exhibiting low-level MYC amplification in the form of homogeneously staining regions (DM(-) cells). Both cell types were studied at genomic and functional levels. The DM(+) cells display a second amplification, corresponding to the 14q24.1 region, in a distinct population of DMs. The 0.43-Mb amplified and overexpressed region contains the PLEK2, PIGH, ARG2, VTI1B, RDH11, and ZFYVE26 genes. Both amplicons were stably maintained upon in vitro and in vivo propagation. However, the 14q24.1 amplicon was not found in cells with high-level MYC amplification in the form of HSRs, either obtained after spontaneous integration of endogenous DM MYC copies or after transfection of DM(-) cells with a MYC gene expression vector. These HSR-bearing cells are highly tumorigenic. The 14q24.1 amplification may not play a role in malignancy per se but might contribute to maintaining the amplification in the form of DMs.
Authors:
M Guillaud-Bataille; O Brison; G Danglot; C Lavialle; B Raynal; V Lazar; P Dessen; A Bernheim
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Publication Detail:
Type:  Journal Article     Date:  2009-04-14
Journal Detail:
Title:  Cytogenetic and genome research     Volume:  124     ISSN:  1424-859X     ISO Abbreviation:  Cytogenet. Genome Res.     Publication Date:  2009  
Date Detail:
Created Date:  2009-04-17     Completed Date:  2009-05-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101142708     Medline TA:  Cytogenet Genome Res     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  1-11     Citation Subset:  IM    
Copyright Information:
Copyright 2009 S. Karger AG, Basel.
Affiliation:
CNRS, FRE2939, Laboratoire de Génomique Cellulaire des Cancers, Institut Gustave Roussy, Villejuif, France.
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MeSH Terms
Descriptor/Qualifier:
Breast Neoplasms / genetics*,  pathology
Carcinoma / genetics*
Cell Line, Tumor
Chromosome Aberrations
Chromosomes, Artificial, Bacterial
Chromosomes, Human, Pair 14*
Chromosomes, Human, Pair 8*
Clone Cells
Cytogenetics
DNA, Neoplasm
Female
Gene Amplification
Genes, myc*
Humans
In Situ Hybridization, Fluorescence
Nucleic Acid Hybridization
Reference Values
Transcription, Genetic
Chemical
Reg. No./Substance:
0/DNA, Neoplasm

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