Document Detail

Two-photon techniques in tissue engineering.
MedLine Citation:
PMID:  20458691     Owner:  NLM     Status:  MEDLINE    
PURPOSE: NIR radiation in the range of about 800 nm is less absorbed by biological tissues and is suited for triggering photonic effects using femtosecond pulsed Ti:Sa lasers. Especially in the life sciences, two-photon techniques are gaining greater importance. We introduce two laser applications for tissue engineering: the autofluorescent visualization of cells seeded on 3D scaffolds after two-photon excitation; and the manufacturing of 3D-structured hydrogel-like scaffolds by triggering free-radical polymerization processes within polymerizable precursors. METHODS: Primary bovine chondrocytes were cultivated on collagen I/III scaffolds using a flow chamber system coupled with a two-photon laser scanning microscope (2PLSM). During the incubation the cell population was hydrostatically stimulated. The selective visualization of unlabeled cells and scaffolds was achieved by spectral autofluorescence imaging. To gain some insight into scaffold-mediated effects on cell growth and cell differentiation, hydrogel-like scaffolds with well defined 3D structures were generated by two-photon polymerization (2PP) using methacrylated urethane and polyethyleneglycol diacrylate. RESULTS: We were able to show that spectral autofluorescence imaging provides spatially resolved data for the non-invasive online control of the tissue engineering process as well as the quantification of cell distribution within the scaffold. The fabrication of 3D 2PP scaffolds made from hydrogel-forming monomers and their effect on cell attachment and cell growth were also shown. CONCLUSIONS: Two-photon techniques provide powerful tools for both the non-invasive online visualization of 3D cell-scaffold constructs and the structuring of 3D cultivation environments. The application of these techniques is also suitable for integration into micro-systems technology (e.g. BioMEMS, Cells-on-Chip, Lab-on-a Chip).
Ronald Schade; Thomas Weiss; Albrecht Berg; Matthias Schnabelrauch; Klaus Liefeith
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The International journal of artificial organs     Volume:  33     ISSN:  0391-3988     ISO Abbreviation:  Int J Artif Organs     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-05-11     Completed Date:  2010-08-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7802649     Medline TA:  Int J Artif Organs     Country:  Italy    
Other Details:
Languages:  eng     Pagination:  219-27     Citation Subset:  IM    
Institute for Bioprocessing and Analytical Measurement Techniques (iba), Department of Biomaterials, Heilbad Heiligenstadt - Germany.
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MeSH Terms
Collagen / metabolism
Imaging, Three-Dimensional / methods*
Tissue Engineering / methods*
Tissue Scaffolds* / chemistry
Reg. No./Substance:
0/Hydrogels; 0/Polymers; 9007-34-5/Collagen

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