Document Detail

Two-photon polymerization technique for microfabrication of CAD-designed 3D scaffolds from commercially available photosensitive materials.
MedLine Citation:
PMID:  18265416     Owner:  NLM     Status:  MEDLINE    
We report on recent advances in the fabrication of three-dimensional (3D) scaffolds for tissue engineering and regenerative medicine constructs using a two-photon polymerization technique (2PP). 2PP is a novel CAD/CAM technology allowing the fabrication of any computer-designed 3D structure from a photosensitive polymeric material. The flexibility of this technology and the ability to precisely define 3D construct geometry allows issues associated with vascularization and patient-specific tissue fabrication to be directly addressed. The fabrication of reproducible scaffold structures by 2PP is important for systematic studies of cellular processes and better understanding of in vitro tissue formation. In this study, 2PP was applied for the generation of 3D scaffold-like structures, using the photosensitive organic-inorganic hybrid polymer ORMOCER (ORganically MOdified CERamics) and epoxy-based SU8 materials. By comparing the proliferation rates of cells grown on flat material surfaces and under control conditions, it was demonstrated that ORMOCER and SU8 are not cytotoxic. Additional tests show that the DNA strand breaking of GFSHR-17 granulosa cells was not affected by the presence of ORMOCER. Furthermore, gap junction conductance measurements revealed that ORMOCER did not alter the formation of cell-cell junctions, critical for functional tissue growth. The possibilities of seeding 3D structures with cells were analysed. These studies demonstrate the great potential of 2PP technique for the manufacturing of scaffolds with controlled topology and properties.
Aleksandr Ovsianikov; Sabrina Schlie; Anaclet Ngezahayo; Axel Haverich; Boris N Chichkov
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of tissue engineering and regenerative medicine     Volume:  1     ISSN:  1932-6254     ISO Abbreviation:  -     Publication Date:    2007 Nov-Dec
Date Detail:
Created Date:  2008-02-18     Completed Date:  2008-06-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101308490     Medline TA:  J Tissue Eng Regen Med     Country:  England    
Other Details:
Languages:  eng     Pagination:  443-9     Citation Subset:  IM    
Laser Zentrum Hannover e.V., Hollerithallee 8, D-30419 Hannover, Germany.
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MeSH Terms
Biocompatible Materials / metabolism*,  pharmacology,  radiation effects
Cell Proliferation / drug effects
Cells, Cultured
Comet Assay
Computer-Aided Design*
DNA Damage
Electric Conductivity
Gap Junctions / metabolism
Granulosa Cells / cytology,  drug effects
Microscopy, Electron, Scanning
Polymers / chemistry,  pharmacology,  radiation effects*
Surface Properties
Time Factors
Tissue Engineering / methods*
Tissue Scaffolds / chemistry*
Reg. No./Substance:
0/Biocompatible Materials; 0/Polymers

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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