| Two-photon irradiation of an intracellular singlet oxygen photosensitizer: achieving localized sub-cellular excitation in spatially-resolved experiments. | |
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MedLine Citation:
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PMID: 20942568 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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The response of a given cell to spatially-resolved sub-cellular irradiation of a singlet oxygen photosensitizer (protoporphyrin IX, PpIX) using a focused laser was assessed. In these experiments, incident light was scattered over a volume greater than that defined by the dimensions of the laser beam as a consequence of the inherent inhomogeneity of the cell. Upon irradiation at a wavelength readily absorbed by PpIX in a one-photon transition, this scattering of light eliminated any advantage accrued to the use of focused irradiation. However, upon irradiation at a longer wavelength where PpIX can only absorb light under non-linear two-photon conditions, meaningful intracellular resolution was achieved in the small spatial domain where the light intensity was high enough for absorption to occur. |
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Authors:
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Brian Wett Pedersen; Thomas Breitenbach; Robert W Redmond; Peter R Ogilby |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-10-13 |
Journal Detail:
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Title: Free radical research Volume: 44 ISSN: 1029-2470 ISO Abbreviation: Free Radic. Res. Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-11-04 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9423872 Medline TA: Free Radic Res Country: England |
Other Details:
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Languages: eng Pagination: 1383-97 Citation Subset: IM |
Affiliation:
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Center for Oxygen Microscopy and Imaging, Department of Chemistry, Aarhus University, DK-8000, Århus, Denmark. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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