| Two-photon imaging during prolonged middle cerebral artery occlusion in mice reveals recovery of dendritic structure after reperfusion. | |
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MedLine Citation:
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PMID: 19005062 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Filament occlusion of the middle cerebral artery (MCA) is a well accepted animal model of focal ischemia. Advantages of the model are relatively long occlusion times and a large penumbra region that simulates aspects of human stroke. Here, we use two-photon and confocal microscopy in combination with regional measurement of blood flow using laser speckle to assess the spatial relationship between the borders of the MCA ischemic territory and loss of dendrite structure, as well as the effect of reperfusion on dendritic damage in adult YFP (yellow fluorescent protein) and GFP (green fluorescent protein) C57BL/6 transgenic mice with fluorescent (predominantly layer 5) neurons. By examining the spatial extent of dendritic damage, we determined that 60 min of MCA occlusion produced a core with severe structural damage that did not recover after reperfusion (begins approximately 3.8 mm lateral to midline), a reversibly damaged area up to 0.6 mm medial to the core that recovered after reperfusion (penumbra), and a relatively structurally intact area ( approximately 1 mm wide; medial penumbra) with hypoperfusion. Loss of structure was preceded by a single ischemic depolarization 122.1 +/- 10.2 s after occlusion onset. Reperfusion of animals after 60 min of ischemia was not associated with exacerbation of damage (reperfusion injury) and resulted in a significant restoration of blebbed dendritic structure, but only within approximately 0.6 mm lateral of the dendritic damage structural border. In summary, we find that recovery of dendritic structure can occur after reperfusion after even 60 min of ischemia, but is likely restricted to a relatively small penumbra region with partial blood flow or oxygenation. |
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Authors:
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Ping Li; Timothy H Murphy |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of neuroscience : the official journal of the Society for Neuroscience Volume: 28 ISSN: 1529-2401 ISO Abbreviation: J. Neurosci. Publication Date: 2008 Nov |
Date Detail:
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Created Date: 2008-11-13 Completed Date: 2009-01-26 Revised Date: 2011-04-28 |
Medline Journal Info:
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Nlm Unique ID: 8102140 Medline TA: J Neurosci Country: United States |
Other Details:
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Languages: eng Pagination: 11970-9 Citation Subset: IM |
Affiliation:
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Kinsmen Laboratory, Department of Psychiatry, University of British Columbia, Vancouver, British Columbia, Canada V6T1Z3. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Brain Infarction / pathology, physiopathology Cell Shape / physiology Cerebrovascular Circulation / physiology Dendrites / pathology, physiology*, ultrastructure Dendritic Spines / pathology, physiology, ultrastructure Disease Models, Animal Green Fluorescent Proteins Infarction, Middle Cerebral Artery / pathology, physiopathology* Luminescent Proteins Male Mice Mice, Inbred C57BL Mice, Transgenic Microscopy, Confocal / methods Microscopy, Fluorescence, Multiphoton / methods* Nerve Degeneration / etiology, pathology, physiopathology* Recovery of Function / physiology* Reperfusion Injury / pathology, physiopathology* |
| Chemical | |
Reg. No./Substance:
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0/Luminescent Proteins; 147336-22-9/Green Fluorescent Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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