| Two-photon excitation of channelrhodopsin-2 at saturation. | |
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MedLine Citation:
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PMID: 19706471 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We demonstrate that channelrhodopsin-2 (CR), a light-gated ion channel that is conventionally activated by using visible-light excitation, can also be activated by using IR two-photon excitation (TPE). An empirical estimate of CR's two-photon absorption cross-section at lambda = 920 nm is presented, with a value (260 +/- 20 GM) indicating that TPE stimulation of CR photocurrents is not typically limited by intrinsic molecular excitability [1 GM = 10(-50)(cm4 s)/photon]. By using direct physiological measurements of CR photocurrents and a model of ground-state depletion, we evaluate how saturation of CR's current-conducting state influences the spatial resolution of focused TPE photostimulation, and how photocurrents stimulated by using low-power scanning TPE temporally summate. We show that TPE, like visible-light excitation, can be used to stimulate action potentials in cultured CR-expressing neurons. |
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Authors:
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John Peter Rickgauer; David W Tank |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. Date: 2009-08-14 |
Journal Detail:
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Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 106 ISSN: 1091-6490 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 2009 Sep |
Date Detail:
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Created Date: 2009-10-06 Completed Date: 2009-11-06 Revised Date: 2010-09-27 |
Medline Journal Info:
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Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: United States |
Other Details:
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Languages: eng Pagination: 15025-30 Citation Subset: IM |
Affiliation:
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Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Line Electrophysiological Phenomena Humans Ion Channels / genetics, metabolism* Patch-Clamp Techniques Photons* Rhodopsin / genetics, metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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1R01MH083686-01/MH/NIMH NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Ion Channels; 9009-81-8/Rhodopsin |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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