| Two-photon absorption of copper tetrasulfophthalocyanine induces phototoxicity towards Jurkat cells in vitro. | |
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MedLine Citation:
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PMID: 17077898 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The feasibility to induce oxygen-independent tumour cell kill by two-photon excitation of copper tetrasulfophthalocyanine (CuPcS4) was studied in Jurkat cells in vitro. Following incubation with CuPcS4 cells were transferred to a closed cuvette and irradiated with 532 nm pulsed-laser or 680 nm continuous-laser light to evaluate the effect of either two- or one-photon excitation, respectively. Cell survival was measured using MTT and Trypan Blue exclusion tests. Cell viability decreased 10-20% following two-photon excitation while one-photon illumination did not affect cell survival. These data confirm that two-photon excitation of CuPcS4 to the upper excited triplet state results in the formation of toxic species suggesting its potential use as a sensitizer for the photodynamic treatment of poorly oxygenated tumours. |
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Authors:
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Youssef Mir; Daniel Houde; Johan E van Lier |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't Date: 2006-09-20 |
Journal Detail:
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Title: Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology Volume: 5 ISSN: 1474-905X ISO Abbreviation: Photochem. Photobiol. Sci. Publication Date: 2006 Nov |
Date Detail:
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Created Date: 2006-11-01 Completed Date: 2007-07-10 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101124451 Medline TA: Photochem Photobiol Sci Country: England |
Other Details:
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Languages: eng Pagination: 1024-30 Citation Subset: IM |
Affiliation:
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Department of Nuclear Medicine and Radiobiology, Faculty of Medicine and Health Sciences, Université de Sherbrooke, Québec, CanadaJ1H 5N4. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Humans Indoles / chemistry*, toxicity* Jurkat Cells Photochemotherapy Photons Photosensitizing Agents / chemistry*, toxicity* Spectrometry, Fluorescence |
| Chemical | |
Reg. No./Substance:
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0/Indoles; 0/Photosensitizing Agents; 33308-41-7/tetrasulfophthalocyanine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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