| Two-photon NADH imaging exposes boundaries of oxygen diffusion in cortical vascular supply regions. | |
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MedLine Citation:
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PMID: 20859293 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Oxygen transport imposes a possible constraint on the brain's ability to sustain variable metabolic demands, but oxygen diffusion in the cerebral cortex has not yet been observed directly. We show that concurrent two-photon fluorescence imaging of endogenous nicotinamide adenine dinucleotide (NADH) and the cortical microcirculation exposes well-defined boundaries of tissue oxygen diffusion in the mouse cortex. The NADH fluorescence increases rapidly over a narrow, very low pO(2) range with a p(50) of 3.4 ± 0.6 mm Hg, thereby establishing a nearly binary reporter of significant, metabolically limiting hypoxia. The transient cortical tissue boundaries of NADH fluorescence exhibit remarkably delineated geometrical patterns, which define the limits of tissue oxygen diffusion from the cortical microcirculation and bear a striking resemblance to the ideal Krogh tissue cylinder. The visualization of microvessels and their regional contribution to oxygen delivery establishes penetrating arterioles as major oxygen sources in addition to the capillary network and confirms the existence of cortical oxygen fields with steep microregional oxygen gradients. Thus, two-photon NADH imaging can be applied to expose vascular supply regions and to localize functionally relevant microregional cortical hypoxia with micrometer spatial resolution. |
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Authors:
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Karl A Kasischke; Elton M Lambert; Ben Panepento; Anita Sun; Harris A Gelbard; Robert W Burgess; Thomas H Foster; Maiken Nedergaard |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2010-09-22 |
Journal Detail:
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Title: Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism Volume: 31 ISSN: 1559-7016 ISO Abbreviation: J. Cereb. Blood Flow Metab. Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2011-01-04 Completed Date: 2011-02-03 Revised Date: 2012-01-12 |
Medline Journal Info:
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Nlm Unique ID: 8112566 Medline TA: J Cereb Blood Flow Metab Country: United States |
Other Details:
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Languages: eng Pagination: 68-81 Citation Subset: IM |
Affiliation:
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Department of Neurology, Center for Neural Development and Disease, University of Rochester Medical Center, Rochester, New York 14642, USA. karl_kasischke@urmc.rochester.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Absorptiometry, Photon Algorithms Animals Cell Hypoxia / physiology Cerebral Angiography Cerebral Cortex / blood supply*, metabolism* Cerebrovascular Circulation / physiology* Diffusion Electrophysiological Phenomena Hypoxia, Brain / pathology Image Processing, Computer-Assisted Mice Mice, Inbred C57BL Microcirculation / physiology NAD / metabolism* Oxygen Consumption / physiology* Physical Stimulation Pia Mater / anatomy & histology, blood supply Somatosensory Cortex / anatomy & histology, metabolism Spectrometry, Fluorescence Vibrissae / physiology |
| Grant Support | |
ID/Acronym/Agency:
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CA68409/CA/NCI NIH HHS; NS38073/NS/NINDS NIH HHS; NS50315/NS/NINDS NIH HHS; P01 NS050315-04/NS/NINDS NIH HHS; R01 CA068409-16/CA/NCI NIH HHS; R01 CA068409-17/CA/NCI NIH HHS; R01 NS038073-13/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
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53-84-9/NAD |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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