Document Detail

Two phosphatidylethanol classes separated by thin layer chromatography are produced by phospholipase D in rat brain hippocampal slices.
MedLine Citation:
PMID:  8814309     Owner:  NLM     Status:  MEDLINE    
Noradrenaline- and ionomycin-stimulated as well as basal phospholipase D activity from rat hippocampus produced, in the presence of ethanol, two different classes of [32P]phosphatidylethanol (designated I and II), which were separated by thin layer chromatography. Endogenous labeling experiments using 3H-fatty acids showed that two different classes of phosphatidylcholine, separated by two-dimensional TLC, one enriched with high incorporation of [3H]arachidonic acid (B) and the other with [3H]myristic acid (A), were the most likely sources for the two classes of phosphatidylethanol. Experiments where individual 32P-phospholipids extracted from [32P]Pi-labeled hippocampal slices were incubated with cabbage phospholipase D, in the presence of ethanol, showed that each class of [32P]phosphatidylcholine, i.e. A and B, produced a different band of [32P]phosphatidylethanol, with the same mobility in TLC as phosphatidylethanol II and I, respectively.
E Sarri; J M Servitja; F Picatoste; E Claro
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEBS letters     Volume:  393     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  1996 Sep 
Date Detail:
Created Date:  1996-11-07     Completed Date:  1996-11-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  303-6     Citation Subset:  IM    
Departament de Bioquimica i Biologia Molecular, Facultat de Medicina, Universitat Autònoma de Barcelona, Bellaterra, Spain.
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MeSH Terms
Arachidonic Acid / metabolism
Chromatography, Thin Layer
Ethanol / metabolism
Fatty Acids / analysis
Hippocampus / enzymology*
Myristic Acid
Myristic Acids / metabolism
Phosphates / metabolism
Phosphatidic Acids / isolation & purification,  metabolism*
Phosphatidylcholines / isolation & purification,  metabolism
Phospholipase D / metabolism*
Phosphorus Radioisotopes
Reg. No./Substance:
0/Fatty Acids; 0/Glycerophospholipids; 0/Myristic Acids; 0/Phosphates; 0/Phosphatidic Acids; 0/Phosphatidylcholines; 0/Phosphorus Radioisotopes; 0/phosphatidylethanol; 10028-17-8/Tritium; 506-32-1/Arachidonic Acid; 544-63-8/Myristic Acid; 64-17-5/Ethanol; EC D

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