Document Detail


Two-phase appearance of oral epithelial dysplasia resulting from focal proliferation of parabasal cells and apoptosis of prickle cells.
MedLine Citation:
PMID:  15689227     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: One of the histologic characteristics of epithelial dysplasias of the oral mucosa is droplet-shaped rete processes resulting from a solid proliferation of basaloid cells. These basaloid cells are suddenly changed into an overlay of parakeratotic cells. However, it is unknown how this characteristic two-phase appearance is generated. METHODS: Formalin-fixed paraffin sections of the oral mucosal specimens with normal, hyperplastic, dysplastic epithelia and squamous cell carcinomas were examined for apoptosis by terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick-end labeling (TUNEL) method and for lymphoid cells by immunohistochemistry. RESULTS: Apoptotic cells were only located in the keratinized layer of normal/hyperplastic epithelia. However, in epithelial dysplasias, apoptotic cells were scattered in the middle or even in the lower parts of the epithelial layer with frequent vacuolation changes of epithelial cells. Within the epithelial layer of dysplasias, there were increased number of lymphocytes, which were immunopositive for CD45RO, CD8, and CD57- and CD68-immunopositive (+), S-100 protein-positive and major histocompatibility complex (MHC) class II-positive monocytic lineages. They increased in number with the severity of dysplastic degrees, and they were often located in the vicinity of apoptotic epithelial cells, but decreased in carcinomas in situ and invasive carcinomas, which contained fewer numbers of apoptotic figures. CONCLUSION: The findings indicate that intraepithelial infiltrations of both cytotoxic T cells and natural killer cells are closely related to the apoptotic phenomena of prickle cells, which may result in the characteristic 'two-phase appearance' of epithelial dysplasia.
Authors:
Mei Syafriadi; Jun Cheng; Kai Yu Jen; Hiroko Ida-Yonemochi; Makoto Suzuki; Takashi Saku
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology     Volume:  34     ISSN:  0904-2512     ISO Abbreviation:  J. Oral Pathol. Med.     Publication Date:  2005 Mar 
Date Detail:
Created Date:  2005-02-03     Completed Date:  2005-03-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8911934     Medline TA:  J Oral Pathol Med     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  140-9     Citation Subset:  D; IM    
Affiliation:
Division of Oral Pathology, Department of Tissue Regeneration and Reconstruction, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
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MeSH Terms
Descriptor/Qualifier:
Apoptosis
CD8-Positive T-Lymphocytes / pathology
Carcinoma in Situ / pathology
Carcinoma, Squamous Cell / pathology
Cell Proliferation
Cell Transformation, Neoplastic / pathology
Epithelial Cells / classification,  pathology
Histocompatibility Antigens Class II / analysis
Humans
Hyperplasia
Killer Cells, Natural / pathology
Lymphocyte Subsets / pathology
Macrophages / pathology
Monocytes / pathology
Mouth Mucosa / pathology*
Mouth Neoplasms / pathology*
Neoplasm Invasiveness
Parakeratosis / pathology
Precancerous Conditions / pathology*
S100 Proteins / analysis
T-Lymphocytes, Cytotoxic / pathology
Vacuoles / ultrastructure
Chemical
Reg. No./Substance:
0/Histocompatibility Antigens Class II; 0/S100 Proteins

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