| Two-parameter mobile phase optimization for the simultaneous high-performance liquid chromatographic determination of dopamine, serotonin and related compounds in microdissected rat brain nuclei. | |
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MedLine Citation:
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PMID: 1716637 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A new high-pressure liquid chromatography method with electrochemical detection is described that allows the simultaneous determination of dopamine, serotonin, 3,4-dihydroxyphenylacetic acid, 3-methoxy-4-hydroxyphenylacetic acid, 5-hydroxytryptophan and 5-hydroxyindoleacetic acid in microdissected nuclei from individual rat brains. No sample pre-treatment steps are required. Resolution and analysis time were optimized by a simple limited optimization procedure, involving two-parameter factorial design. |
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Authors:
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M Lema; J Otero; J Marcó |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of chromatography Volume: 547 ISSN: 0021-9673 ISO Abbreviation: J. Chromatogr. Publication Date: 1991 Jun |
Date Detail:
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Created Date: 1991-10-24 Completed Date: 1991-10-24 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0427043 Medline TA: J Chromatogr Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 113-20 Citation Subset: IM |
Affiliation:
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Department of Physiology, Faculty of Biology, University of Santiago, Galicia, Spain. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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3,4-Dihydroxyphenylacetic Acid
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analysis 5-Hydroxytryptophan / analysis Animals Brain / ultrastructure* Cell Nucleus / chemistry* Chromatography, High Pressure Liquid / methods* Dopamine / analysis* Hydroxyindoleacetic Acid / analysis Male Methoxyhydroxyphenylglycol / analysis Norepinephrine / analysis Rats Rats, Inbred Strains Serotonin / analysis* |
| Chemical | |
Reg. No./Substance:
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102-32-9/3,4-Dihydroxyphenylacetic Acid; 50-67-9/Serotonin; 51-41-2/Norepinephrine; 534-82-7/Methoxyhydroxyphenylglycol; 54-16-0/Hydroxyindoleacetic Acid; 56-69-9/5-Hydroxytryptophan |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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