Document Detail


Two oppositely oriented arrays of low-affinity recognition sites in oriC guide progressive binding of DnaA during Escherichia coli pre-RC assembly.
MedLine Citation:
PMID:  21895796     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The onset of chromosomal DNA replication requires highly precise and reproducible interactions between initiator proteins and replication origins to assemble a pre-replicative complex (pre-RC) that unwinds the DNA duplex. In bacteria, initiator protein DnaA, bound to specific high- and low-affinity recognition sites within the unique oriC locus, comprises the pre-RC, but how complex assembly is choreographed to ensure precise initiation timing during the cell cycle is not well understood. In this study, we present evidence that higher-order DnaA structures are formed at oriC when DnaA monomers are closely positioned on the same face of the DNA helix by interaction with two oppositely oriented essential arrays of closely spaced low-affinity DnaA binding sites. As DnaA levels increase, peripheral high-affinity anchor sites begin cooperative loading of the arrays, which is extended by sequential binding of additional DnaA monomers resulting in growth of the complexes towards the centre of oriC. We suggest that this polarized assembly of unique DnaA oligomers within oriC plays an important role in mediating pre-RC activity and may be a feature found in all bacterial replication origins.
Authors:
Tania A Rozgaja; Julia E Grimwade; Maryam Iqbal; Christopher Czerwonka; Mansi Vora; Alan C Leonard
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2011-09-14
Journal Detail:
Title:  Molecular microbiology     Volume:  82     ISSN:  1365-2958     ISO Abbreviation:  Mol. Microbiol.     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2011-10-12     Completed Date:  2012-01-24     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  8712028     Medline TA:  Mol Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  475-88     Citation Subset:  IM    
Copyright Information:
© 2011 Blackwell Publishing Ltd.
Affiliation:
Department of Biological Sciences, Florida Institute of Technology, 150 W. University Blvd., Melbourne, FL 32901, USA.
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MeSH Terms
Descriptor/Qualifier:
Bacterial Proteins / chemistry,  genetics,  metabolism*
Base Sequence
Binding Sites
DNA Replication*
DNA-Binding Proteins / chemistry,  genetics,  metabolism*
Escherichia coli / chemistry,  genetics,  metabolism*
Molecular Sequence Data
Origin Recognition Complex / chemistry,  genetics,  metabolism*
Protein Binding
Replication Origin*
Grant Support
ID/Acronym/Agency:
R01 GM054042/GM/NIGMS NIH HHS; R01 GM054042-13/GM/NIGMS NIH HHS; R01 GM054042-14/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/DNA-Binding Proteins; 0/DnaA protein, Bacteria; 0/Origin Recognition Complex
Comments/Corrections

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