Document Detail


Two non-identical heads of myosin molecules.
MedLine Citation:
PMID:  3007447     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Four different preparations of skeletal subfragment-1, denoted in this report as S1(Aa), S1(Ab), S1(Ba), and S1(Bb), and two different preparations of cardiac subfragment-1, denoted as S1(A) and S1(B), were obtained as described in our recent report (J. Biochem. 97, 965, 1985). (i) The four preparations were obtained from chicken breast myosin trinitrophenylated with 2,4,6-trinitrobenzene sulfonate in the absence of inorganic pyrophosphate (-PPi), and they were all shown to be trinitrophenylated. Addition of PPi caused change in the absorption spectra of trinitrophenyl(TNP)-S1(Aa) and TNP-S1(Ba), but not in those of TNP-S1(Ab) and TNP-S1(Bb). (ii) The two preparations of S1 were obtained from cardiac myosin trinitrophenylated either in the absence (-) or presence (+) of PPi. S1(B) was trinitrophenylated, whereas S1(A) was not. Specifically emphasized is the observation that the yield of cardiac S1(A) was practically equal to that of cardiac S1(B). On the basis of these results, we propose the hypothesis of "two iso-myosins with non-identical heads," which is essentially a combination of the hypothesis of isoenzymes and that of non-identical heads.
Authors:
T Eiki; S Nakata; N Tanaka; S Ikeda; T Kaneko; A Maruyama; S Watanabe
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of biochemistry     Volume:  99     ISSN:  0021-924X     ISO Abbreviation:  J. Biochem.     Publication Date:  1986 Jan 
Date Detail:
Created Date:  1986-05-15     Completed Date:  1986-05-15     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0376600     Medline TA:  J Biochem     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  33-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphate / analysis
Animals
Chickens
Diphosphates
Hydrolysis
Muscles / analysis
Myocardium / analysis
Myosin Subfragments
Myosins / analysis*
Peptide Fragments / analysis*
Spectrophotometry
Swine
Trinitrobenzenesulfonic Acid
Chemical
Reg. No./Substance:
0/Diphosphates; 0/Myosin Subfragments; 0/Peptide Fragments; 2508-19-2/Trinitrobenzenesulfonic Acid; 56-65-5/Adenosine Triphosphate; EC 3.6.4.1/Myosins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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