Document Detail


Two modes of DNA double-strand break repair are reciprocally regulated through the fission yeast cell cycle.
MedLine Citation:
PMID:  15371339     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Several considerations suggest that levels of the two major modes of double-strand break (DSB) repair, homologous recombination (HR), and nonhomologous end joining (NHEJ), are regulated through the cell cycle. However, this idea has not been explicitly tested. In the absence of the telomere-binding protein Taz1, fission yeast undergo lethal telomere fusions via NHEJ. These fusions occur only during periods of nitrogen starvation and fail to accumulate during logarithmic growth, when the majority of cells are in G2. We show that G1 arrest is the specific nitrogen starvation-induced event that promotes NHEJ between taz1(-) telomeres. Furthermore, the general levels of NHEJ and HR are reciprocally regulated through the cell cycle, so that NHEJ is 10-fold higher in early G1 than in other cell cycle stages; the reverse is true for HR. Whereas NHEJ is known to be dispensable for survival of DSBs in cycling cells, we find that it is critical for repair and survival of DSBs arising during G1.
Authors:
Miguel Godinho Ferreira; Julia Promisel Cooper
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Genes & development     Volume:  18     ISSN:  0890-9369     ISO Abbreviation:  Genes Dev.     Publication Date:  2004 Sep 
Date Detail:
Created Date:  2004-09-16     Completed Date:  2004-11-01     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  8711660     Medline TA:  Genes Dev     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2249-54     Citation Subset:  IM    
Affiliation:
Telomere Biology Laboratory, Cancer Research UK, London WC2A 3PX, UK.
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MeSH Terms
Descriptor/Qualifier:
Cell Cycle / genetics*
Cell Division
DNA / genetics,  metabolism*
DNA Repair / physiology*
G1 Phase / genetics,  radiation effects
G2 Phase / genetics
Gamma Rays
Nitrogen / metabolism
Recombination, Genetic
Schizosaccharomyces / genetics*,  radiation effects
Schizosaccharomyces pombe Proteins / genetics,  metabolism
Telomere / genetics
Telomere-Binding Proteins / genetics,  metabolism
Chemical
Reg. No./Substance:
0/Schizosaccharomyces pombe Proteins; 0/Telomere-Binding Proteins; 0/taz1 protein, S pombe; 7727-37-9/Nitrogen; 9007-49-2/DNA
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