| Two methods for determining plasma IL-6 in humans at rest and following exercise. | |
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MedLine Citation:
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PMID: 18797920 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We compared two methods to determine IL-6 at rest and following exercise. On two occasions, plasma IL-6 was determined in 10 men and 13 women before and immediately following 90 min of cycling at 65% V(O)(2max) using a cytometric bead array (CBA) and an ELISA. The CBA detected IL-6 in 63% of pre-exercise samples and 84% of post-exercise samples; the ELISA detected IL-6 in all samples. In samples with detectable IL-6, resting values were comparable between the CBA and ELISA (2.3 +/- 1.0 and 2.4 +/- 1.2 pg/mL, respectively; p = 0.99); the IL-6 response to exercise was underestimated (p = 0.0003) by the CBA by ~45%, compared with the ELISA. CBA- and ELISA-determined IL-6 values were moderately correlated (r = 0.66, p < 0.0001), but exhibited poor agreement (mean bias +/- 1SD = -1.4 +/- 2.1 pg/mL). The CBA provided concordant IL-6 concentrations at rest, when detected, but underestimated exercise-induced increases in IL-6 versus the ELISA method. |
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Authors:
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Brian W Timmons; Mazen J Hamadeh; Mark A Tarnopolsky |
Publication Detail:
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Type: Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't Date: 2008-09-17 |
Journal Detail:
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Title: European journal of applied physiology Volume: 105 ISSN: 1439-6327 ISO Abbreviation: Eur. J. Appl. Physiol. Publication Date: 2009 Jan |
Date Detail:
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Created Date: 2009-01-09 Completed Date: 2009-03-26 Revised Date: 2009-04-08 |
Medline Journal Info:
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Nlm Unique ID: 100954790 Medline TA: Eur J Appl Physiol Country: Germany |
Other Details:
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Languages: eng Pagination: 13-8 Citation Subset: IM |
Affiliation:
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Children's Exercise and Nutrition Centre, McMaster University, Chedoke Hospital, Evel Bldg, Sanatorium Road, Hamilton, ON, L8N 3Z5, Canada. timmonbw@mcmaster.ca |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Enzyme-Linked Immunosorbent Assay / methods Exercise / physiology* Female Flow Cytometry / methods* Humans Interleukin-6 / blood* Male Oxygen Consumption / physiology Physical Endurance Rest / physiology* |
| Chemical | |
Reg. No./Substance:
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0/Interleukin-6 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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