Document Detail


Two methods for determining plasma IL-6 in humans at rest and following exercise.
MedLine Citation:
PMID:  18797920     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We compared two methods to determine IL-6 at rest and following exercise. On two occasions, plasma IL-6 was determined in 10 men and 13 women before and immediately following 90 min of cycling at 65% V(O)(2max) using a cytometric bead array (CBA) and an ELISA. The CBA detected IL-6 in 63% of pre-exercise samples and 84% of post-exercise samples; the ELISA detected IL-6 in all samples. In samples with detectable IL-6, resting values were comparable between the CBA and ELISA (2.3 +/- 1.0 and 2.4 +/- 1.2 pg/mL, respectively; p = 0.99); the IL-6 response to exercise was underestimated (p = 0.0003) by the CBA by ~45%, compared with the ELISA. CBA- and ELISA-determined IL-6 values were moderately correlated (r = 0.66, p < 0.0001), but exhibited poor agreement (mean bias +/- 1SD = -1.4 +/- 2.1 pg/mL). The CBA provided concordant IL-6 concentrations at rest, when detected, but underestimated exercise-induced increases in IL-6 versus the ELISA method.
Authors:
Brian W Timmons; Mazen J Hamadeh; Mark A Tarnopolsky
Publication Detail:
Type:  Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-09-17
Journal Detail:
Title:  European journal of applied physiology     Volume:  105     ISSN:  1439-6327     ISO Abbreviation:  Eur. J. Appl. Physiol.     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2009-01-09     Completed Date:  2009-03-26     Revised Date:  2009-04-08    
Medline Journal Info:
Nlm Unique ID:  100954790     Medline TA:  Eur J Appl Physiol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  13-8     Citation Subset:  IM    
Affiliation:
Children's Exercise and Nutrition Centre, McMaster University, Chedoke Hospital, Evel Bldg, Sanatorium Road, Hamilton, ON, L8N 3Z5, Canada. timmonbw@mcmaster.ca
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MeSH Terms
Descriptor/Qualifier:
Adult
Enzyme-Linked Immunosorbent Assay / methods
Exercise / physiology*
Female
Flow Cytometry / methods*
Humans
Interleukin-6 / blood*
Male
Oxygen Consumption / physiology
Physical Endurance
Rest / physiology*
Chemical
Reg. No./Substance:
0/Interleukin-6

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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