| Two mass-spectrometric techniques for quantifying serine enantiomers and glycine in cerebrospinal fluid: potential confounders and age-dependent ranges. | |
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MedLine Citation:
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PMID: 18606633 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: The recent discovery and specific functions of D-amino acids in humans are bound to lead to the revelation of D-amino acid abnormalities in human disorders. Therefore, high-throughput analysis techniques are warranted to determine D-amino acids in biological fluids in a routine laboratory setting. METHODS: We developed 2 chromatographic techniques, a nonchiral derivatization with chiral (chirasil-L-val column) separation in a GC-MS system and a chiral derivatization with Marfey's reagent and LC- MS analysis. We validated the techniques for D-serine, L-serine, and glycine determination in cerebrospinal fluid (CSF), evaluated several confounders, and determined age-dependent human concentration ranges. RESULTS: Quantification limits for D-serine, L-serine, and glycine in cerebrospinal fluid were 0.14, 0.44, and 0.14 micromol/L, respectively, for GC-MS and 0.20, 0.41, and 0.14 micromol/L for LC-MS. Within-run imprecision was <3% for both methods, and between-run imprecision was <13%. Comparison of both techniques with Deming regression yielded coefficients of 0.90 (D-serine), 0.92 (L-serine), and 0.96 (glycine). Sample collection, handling, and transport is uncomplicated-there is no rostrocaudal CSF gradient, no effect of storage at 4 degrees C for 1 week before storage at -80 degrees C, and no effect of up to 3 freeze/thaw cycles. Conversely, contamination with erythrocytes increased D-serine, L-serine, and glycine concentrations. CSF concentrations for 145 apparently healthy controls demonstrated markedly and specifically increased (5 to 9 times) D-serine concentrations during early central nervous system development. CONCLUSIONS: These 2 clinically applicable analysis techniques will help to unravel pathophysiologic, diagnostic, and therapeutic issues for disorders associated with central nervous system abnormalities, NMDA-receptor dysfunction, and other pathology associated with D-amino acids. |
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Authors:
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Sabine A Fuchs; Monique G M de Sain-van der Velden; Martina M J de Barse; Martin W Roeleveld; Margriet Hendriks; Lambertus Dorland; Leo W J Klomp; Ruud Berger; Tom J de Koning |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-07-07 |
Journal Detail:
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Title: Clinical chemistry Volume: 54 ISSN: 1530-8561 ISO Abbreviation: Clin. Chem. Publication Date: 2008 Sep |
Date Detail:
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Created Date: 2008-08-29 Completed Date: 2008-09-12 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9421549 Medline TA: Clin Chem Country: United States |
Other Details:
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Languages: eng Pagination: 1443-50 Citation Subset: IM |
Affiliation:
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Department of Metabolic and Endocrine Diseases, University Medical Center Utrecht, Utrecht, The Netherlands. S.Fuchs@umcutrecht.nl |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adult Cerebrospinal Fluid / chemistry* Child Child, Preschool Glycine / chemistry Health Humans Infant Infant, Newborn Mass Spectrometry / methods* Serine / chemistry Stereoisomerism |
| Chemical | |
Reg. No./Substance:
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56-40-6/Glycine; 56-45-1/Serine |
| Comments/Corrections | |
Comment In:
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Clin Chem. 2008 Sep;54(9):1413-4
[PMID:
18755902
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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