Document Detail


Two-log increase in sensitivity for detection of norovirus in complex samples by concentration with porcine gastric mucin conjugated to magnetic beads.
MedLine Citation:
PMID:  18515489     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human histo-blood group antigens (HBGA) have been identified previously as candidate receptors for human norovirus (NOR). Type A, type H1, and Lewis HBGA in humans have been identified as major HBGA for NOR binding. We have found that pig stomach (gastric) mucin (PGM) contains blood group A, H1, and Lewis b HBGA and binds to multiple strains of NOR more broadly than do specific antibodies to NOR. Both genogroup I (GGI) and GGII NOR strains were recovered by PGM-conjugated magnetic beads. A fecal sample containing GGII NOR was detected at a dilution of 1:1,000,000 by the standard RNA extraction procedure, whereas NOR in a 1:100,000,000 dilution could be concentrated by PGM-conjugated magnetic beads and NOR in spiked food samples (e.g., oyster extract, strawberry, raspberry, and lettuce) was captured by PGM, thus minimizing the reverse transcription-PCR inhibitors in food and increasing sensitivity.
Authors:
Peng Tian; Anna Engelbrektson; Robert Mandrell
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2008-05-30
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  74     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2008 Jul 
Date Detail:
Created Date:  2008-07-14     Completed Date:  2008-07-31     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4271-6     Citation Subset:  IM    
Affiliation:
Produce Safety and Microbiology Research Unit, WRRC, ARS, USDA, 800 Buchanan Street, Albany, CA 94710-1105, USA. ptian@pw.usda.gov
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood Group Antigens / metabolism
Crassostrea / virology
Feces / virology*
Food Microbiology
Fragaria / virology
Gastric Mucins / metabolism*
Humans
Immunomagnetic Separation / methods*
Lettuce / virology
Microspheres
Norovirus / isolation & purification*
RNA, Viral / isolation & purification
Reverse Transcriptase Polymerase Chain Reaction
Sensitivity and Specificity
Sus scrofa
Chemical
Reg. No./Substance:
0/Blood Group Antigens; 0/Gastric Mucins; 0/RNA, Viral
Comments/Corrections

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