Document Detail

Two lines of MDCK epithelial cells with different volume and ion responses to calcium ionophore A23187.
MedLine Citation:
PMID:  2839464     Owner:  NLM     Status:  MEDLINE    
Madin-Darby canine kidney (MDCK) cells lost volume when exposed to 50 microM calcium ionophore A23187. The ionic mechanism for the volume reduction differed between two types of cells. MDCK1 lost potassium, similar to cells undergoing a volume regulatory decrease in hypotonic medium. MDCK2 predominately lost sodium, as did cells with elevated cyclic AMP or when treated with amiloride. The effects of A23187 and dibutyryl-cyclic AMP on Na and K content and volume suggest that intracellular second messengers can trigger different mechanisms to reduce cell volume.
J C Allen; J W Mills
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  In vitro cellular & developmental biology : journal of the Tissue Culture Association     Volume:  24     ISSN:  0883-8364     ISO Abbreviation:  In Vitro Cell. Dev. Biol.     Publication Date:  1988 Jun 
Date Detail:
Created Date:  1988-08-17     Completed Date:  1988-08-17     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8506951     Medline TA:  In Vitro Cell Dev Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  588-92     Citation Subset:  IM    
Department of Anatomy, Dartmouth Medical School, Hanover, New Hampshire 03756.
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MeSH Terms
Amiloride / pharmacology
Bucladesine / pharmacology
Calcimycin / pharmacology*
Cell Line
Cyclic AMP / metabolism
Epithelial Cells
Epithelium / metabolism
Hypotonic Solutions
Kidney / cytology*,  drug effects,  metabolism
Potassium / metabolism
Sodium / metabolism
Grant Support
Reg. No./Substance:
0/Hypotonic Solutions; 2609-46-3/Amiloride; 362-74-3/Bucladesine; 52665-69-7/Calcimycin; 60-92-4/Cyclic AMP; 7440-09-7/Potassium; 7440-23-5/Sodium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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