Document Detail


Two lepidopteran cell lines stably transformed by the abc transporter gene pdr5 show tolerance to diacetoxyscirpenol.
MedLine Citation:
PMID:  16618208     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The pleiotropic drug resistance 5 gene (pdr5) encodes a multidrug membrane transporter and plays a very important role in the efflux of a broad range of chemicals in yeast cells. To study the possible function of pdr5 in insect cells, two stably pdr5-transformed lepidopteran insect cell lines, Sf21 and CF-203, were developed. Transcripts of pdr5 were detected in these two lines using Northern blotting and RT-PCR analysis. When cells were treated with the protein synthesis inhibitor diacetoxyscirpenol, the transformed Sf21 and CF-203 cell lines showed increased tolerance to this chemical. However, unlike in yeast cells, ecdysone agonist RH5992 could not be excluded by PDR5, probably because of low expression levels or imperfect incorporation of the recombinant protein in these transformed cell lines.
Authors:
D-Y Zhang; P J Krell; Q-L Feng
Related Documents :
19377508 - Overexpression of an activated rel mutant enhances the transformed state of the human b...
3829318 - Morphological transformation in three mammalian cell systems following treatment with 6...
2122238 - Altered transcriptional activity of c-fos promoter plasmids in v-raf-transformed nih 3t...
6318968 - Lack of reverse transformation after treatment of a 549 human alveolar carcinoma cells ...
12967138 - Overexpression of the r2 subunit of ribonucleotide reductase in human nasopharyngeal ca...
11758888 - Transgenic technology and applications in swine.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  In vitro cellular & developmental biology. Animal     Volume:  42     ISSN:  1071-2690     ISO Abbreviation:  In Vitro Cell. Dev. Biol. Anim.     Publication Date:    2006 Jan-Feb
Date Detail:
Created Date:  2006-04-18     Completed Date:  2006-07-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9418515     Medline TA:  In Vitro Cell Dev Biol Anim     Country:  United States    
Other Details:
Languages:  eng     Pagination:  27-32     Citation Subset:  IM    
Affiliation:
Great Lakes Forestry Centre, Canadian Forest Service, 1219 Queen Street East, Sault Ste. Marie, Ontario, Canada, P6A 2E5.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
ATP-Binding Cassette Transporters* / genetics,  metabolism
Animals
Antineoplastic Agents / metabolism*
Cell Line
Drug Resistance / genetics*
Hydrazines / metabolism
Insecticides / metabolism
Lepidoptera* / cytology,  physiology
Saccharomyces cerevisiae Proteins* / genetics,  metabolism
Transformation, Genetic
Trichothecenes / metabolism*
Chemical
Reg. No./Substance:
0/ATP-Binding Cassette Transporters; 0/Antineoplastic Agents; 0/Hydrazines; 0/Insecticides; 0/PDR5 protein, S cerevisiae; 0/Saccharomyces cerevisiae Proteins; 0/Trichothecenes; 112410-23-8/tebufenozide; 2270-40-8/diacetoxyscirpenol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Ethanolamine modulates DNA synthesis through epidermal growth factor receptor in rat primary hepatoc...
Next Document:  Molecular analysis and characterization of PrEC, commercially available prostate epithelial cells.