Document Detail


Two glycogen synthase activities associated with proteoglycogen in retina.
MedLine Citation:
PMID:  10685612     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Glycogen synthase of bovine retina was found associated with the acid-insoluble and acid-soluble proteoglycogen fractions. The synthase associated with the acid-insoluble proteoglycogen precursor showed an 8-fold lower Km for UDP-glucose than the synthase associated with the acid-soluble fraction, and was inhibited by detergent. A short digestion with pronase resulted in conversion of the acid insoluble fraction into acid-soluble. The results lead us to postulate that the acid-insolubility of the proteoglycogen fraction and the association with retina membrane proposed before, is caused by glycogen synthase strongly associated to its polysaccharide moiety. The enlargement of the polysaccharide moiety during proteoglycogen biosynthesis, from glycogenin linked to a few 11 to 12 glucose units to the acid-insoluble proteoglycogen precursor (Mr 470,000) would be carried out, together with the branching enzyme, by the glycogen synthase showing a low Km for UDP-glucose. The glycogen synthase with the highest Km for UDP-glucose would participate in conversion of the precursor into mature acid-soluble proteoglycogen.
Authors:
J A Curtino; E R Lacoste
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neurochemical research     Volume:  25     ISSN:  0364-3190     ISO Abbreviation:  Neurochem. Res.     Publication Date:  2000 Jan 
Date Detail:
Created Date:  2000-03-09     Completed Date:  2000-03-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7613461     Medline TA:  Neurochem Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  129-32     Citation Subset:  IM    
Affiliation:
Centro de Investigaciones en Química Biológica de Córdoba (CIQUIBIC), UNC-CONICET, Departamento de Química Biológica Dr. Ranwel Caputto, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Argentina. jcurtino@dqbfcq.uncor.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Cattle
Detergents / pharmacology
Enzyme Inhibitors / pharmacology
Glucosyltransferases
Glycogen Synthase / antagonists & inhibitors,  metabolism*
Glycoproteins / chemistry,  metabolism*
Glycosylation
Hydrogen-Ion Concentration
Polysaccharides / chemistry,  metabolism*
Pronase / metabolism
Retina / enzymology*
Solubility
Trichloroacetic Acid
Uridine Diphosphate Glucose / metabolism
Chemical
Reg. No./Substance:
0/Detergents; 0/Enzyme Inhibitors; 0/Glycoproteins; 0/Polysaccharides; 0/glycogenin; 133-89-1/Uridine Diphosphate Glucose; 76-03-9/Trichloroacetic Acid; EC 2.4.1.-/Glucosyltransferases; EC 2.4.1.11/Glycogen Synthase; EC 3.4.24.-/Pronase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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