Document Detail

Two endocytic recycling routes selectively fill two vesicle pools in frog motor nerve terminals.
MedLine Citation:
PMID:  11055437     Owner:  NLM     Status:  MEDLINE    
We have identified and characterized two vesicle recycling pathways in frog motor nerve terminals. We exploited the differential staining properties of FM dyes of varying hydrophobicity to label selectively two different vesicle pools, using optical imaging and electron microscopy of photoconverted dyes. During a 1 min tetanus, a rapidly recycling route places vesicles selectively into a small readily releasable pool comprising about 20% of vesicles. After the tetanus, a much slower pathway (from which FM2-10 but not FM1-43 can be rinsed) delivers vesicles via infoldings and cisternae selectively to a reserve pool with a halftime of about 8 min. Mixing between the two pools is slow. During stimulation at 30 Hz, 10-15 s is required to mobilize and release dye from the reserve pool.
D A Richards; C Guatimosim; W J Betz
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Neuron     Volume:  27     ISSN:  0896-6273     ISO Abbreviation:  Neuron     Publication Date:  2000 Sep 
Date Detail:
Created Date:  2000-10-30     Completed Date:  2000-11-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8809320     Medline TA:  Neuron     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  551-9     Citation Subset:  IM    
Department of Physiology and Biophysics, University of Colorado Medical School, Denver 80262, USA.
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MeSH Terms
Endocytosis / physiology*
Fluorescent Dyes / pharmacokinetics
Motor Neurons / metabolism*
Muscle Contraction / physiology
Neuromuscular Junction / metabolism*,  ultrastructure
Pyridinium Compounds / pharmacokinetics
Quaternary Ammonium Compounds / pharmacokinetics
Rana pipiens
Synaptic Vesicles / metabolism*
Reg. No./Substance:
0/FM1 43; 0/FM2 10; 0/Fluorescent Dyes; 0/Pyridinium Compounds; 0/Quaternary Ammonium Compounds

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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