| Two domains with amino-acid sequence similarity are required for dihydroneopterin aldolase function in the multifunctional folic acid synthesis Fas protein of Pneumocystis carinii. | |
| | |
MedLine Citation:
|
PMID: 7543066 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The folic acid synthesized gene (fas) of Pneumocystis carinii (Pc) codes for a multifunctional enzyme (Fas) known to catalyse three consecutive steps leading to the production of dihydropteroate in the de novo folate synthesis pathway. Previously, we predicted that a domain, designated FasB (amino acids (aa) 161-280), of the 740-aa multifunctional protein contains the first of the three enzyme activities in the pathway, namely dihydroneopterin aldolase (DHNA), since it shares 23% aa identity with the DHNA of Streptococcus pneumoniae (Sp). We now extend these findings to show that a second domain, FasA (aa 39-160), whose function was previously unknown, shares 27% sequence identity with the adjacent FasB domain, indicative of functional similarity. FasA is also 18% identical with the DHNA from Sp. Recombinant baculoviruses were constructed which directed the production of either FasA, FasB or FasAB polypeptide species in cultured Spodoptera frugiperda (SF9) insect cells. No DHNA activity is associated with either fasA or fasB when produced as single domains in the insect-baculovirus system. However, DHNA activity was detected in SF9 extracts containing the overproduced FasAB polypeptide. The results of aa sequence alignments and expression studies suggest that FasA and FasB may be two subunits of the DHNA enzyme moiety within the multifunctional Fas protein of Pc. An alternative interpretation of the results is also discussed. |
| | |
Authors:
|
F Volpe; S P Ballantine; C J Delves |
Related Documents
:
|
999896 - Isolation, properties and primary structure of coypu and chinchilla pancreatic ribonucl... 11902666 - Primary structure characterization of bothrops jararacussu snake venom lectin. 9349716 - Cloning of the phytases from emericella nidulans and the thermophilic fungus talaromyce... 23664936 - Characterization of esterified cassava starch with long alkyl side chains and different... 15574476 - In vitro activity of bergamot natural essence and furocoumarin-free and distilled extra... 16953466 - Antihyperuricemic lignans from the leaves of phyllanthus niruri. |
Publication Detail:
|
Type: Comparative Study; Journal Article |
Journal Detail:
|
Title: Gene Volume: 160 ISSN: 0378-1119 ISO Abbreviation: Gene Publication Date: 1995 Jul |
Date Detail:
|
Created Date: 1995-09-07 Completed Date: 1995-09-07 Revised Date: 2009-09-29 |
Medline Journal Info:
|
Nlm Unique ID: 7706761 Medline TA: Gene Country: NETHERLANDS |
Other Details:
|
Languages: eng Pagination: 41-6 Citation Subset: IM |
Affiliation:
|
Structural Biology Group, Wellcome Research Laboratories, Beckenham, Kent, UK. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Aldehyde-Lyases
/
genetics,
metabolism* Amino Acid Sequence Animals Antigens, CD95 Antigens, Surface / genetics, metabolism* Base Sequence Blotting, Western Cell Line DNA Primers Folic Acid / biosynthesis Fungal Proteins / genetics, metabolism* Genes, Fungal Kinetics Molecular Sequence Data Open Reading Frames Pneumocystis / enzymology*, genetics Recombinant Proteins / metabolism Sequence Homology, Amino Acid Spodoptera Streptococcus pneumoniae / genetics Transfection |
| Grant Support | |
ID/Acronym/Agency:
|
//Wellcome Trust |
| Chemical | |
Reg. No./Substance:
|
0/Antigens, CD95; 0/Antigens, Surface; 0/DNA Primers; 0/FasA protein, Pneumocystis; 0/FasB protein, Pneumocystis; 0/Fungal Proteins; 0/Recombinant Proteins; 59-30-3/Folic Acid; EC 4.1.2.-/Aldehyde-Lyases; EC 4.1.2.25/dihydroneopterin aldolase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Regulation of angR, a gene with regulatory and biosynthetic functions in the pJM1 plasmid-mediated i...
Next Document: Glutathione-S-transferase activity and multidrug resistance phenotype in chronic lymphocytic leukemi...