| Two distinct cell sources of H2O2 in the lignifying Zinnia elegans cell culture system. | |
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MedLine Citation:
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PMID: 16520879 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The use of transdifferentiating Zinnia elegans mesophyll cells has proved useful in investigations of the process of xylem differentiation from cambial derivatives. Cultured mesophyll cells can be induced by external stimuli to proceed through temporally controlled developmental programs which conclude in the formation of single-cell-derived dead vascular tracheids and parenchyma-like elements. However, there is a gap in our knowledge concerning the role played by reactive oxygen species (O(2) (-) and H(2)O(2)) in the development of these vascular elements. In this study, we show by the following four independent and highly selective methods that transdifferentiating Z. elegans mesophyll cells are capable of producing reactive oxygen species: the 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) assay, which monitors O(2) (-) production, and the xylenol orange, 2,7-dichlorofluorescein diacetate, and CeCl(3) assays, which monitor H(2)O(2) production and localization. The joint use of these biochemical (XTT and xylenol orange) assays and cytochemical (2,7-dichlorofluorescein diacetate and CeCl(3)) probes revealed that transdifferentiating Z. elegans mesophyll cells do not show an oxidative burst but live in a strongly oxidative state during the entire culture period. In this state, H(2)O(2) is produced by both tracheary and parenchyma-like elements, the nonlignifying parenchyma-like cells acting quantitatively as the main source. The existence of these two sources of H(2)O(2) in this in vitro cell culture system may be especially relevant during the later stages of tracheary cell wall lignification, in which lignifying tracheary elements become hollow. In the case of differentiating tracheary elements, H(2)O(2) was located in the same place and at the same time as the onset of tracheary element lignification, i.e., at the primary cell wall during secondary thickening, supporting the view that the H(2)O(2) produced by this in vitro culture system is destined for use during lignin biosynthesis. |
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Authors:
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L V Gómez Ros; A Paradiso; C Gabaldón; M A Pedreño; L de Gara; A Ros Barceló |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-03-09 |
Journal Detail:
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Title: Protoplasma Volume: 227 ISSN: 0033-183X ISO Abbreviation: Protoplasma Publication Date: 2006 May |
Date Detail:
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Created Date: 2006-05-31 Completed Date: 2006-08-02 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9806853 Medline TA: Protoplasma Country: Austria |
Other Details:
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Languages: eng Pagination: 175-83 Citation Subset: IM |
Affiliation:
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Department of Plant Biology, University of Murcia, Murcia, Spain. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Asteraceae
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cytology*,
metabolism*,
ultrastructure Cell Culture Techniques Cell Differentiation Hydrogen Peroxide / metabolism* Lignin / metabolism* Plant Stems / cytology Superoxides / metabolism Time Factors |
| Chemical | |
Reg. No./Substance:
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11062-77-4/Superoxides; 7722-84-1/Hydrogen Peroxide; 9005-53-2/Lignin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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