Document Detail

Two-dimensional electrophoresis of surface glycoproteins of normal BHK cells and ricin resistant mutants.
MedLine Citation:
PMID:  760785     Owner:  NLM     Status:  MEDLINE    
The surface glycoproteins of baby hamster kidney (BHK) cells were iodinated by lactoperoxidase and submitted to a two-dimensional electrophoresis procedure involving isoelectric focusing in the first dimension and SDS gel electrophoresis in the second dimension. After autoradiography a complex but reproducible pattern was obtained. The technique was then applied to the study of three ricin-resistant mutant clones with reduced rates of cell-cell and/or cell-substratum adhesion. Abnormal patterns were observed in all three mutant clones indicating different mechanisms of ricin resistance and identifying glycoproteins which may be involved in cellular interactions.
S D Pena; G Mills; R C Hughes
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  550     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1979 Jan 
Date Detail:
Created Date:  1979-04-25     Completed Date:  1979-04-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  100-9     Citation Subset:  IM    
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MeSH Terms
Cell Adhesion
Cell Communication
Cell Line
Cell Membrane / drug effects,  metabolism*
Drug Resistance
Glycoproteins / isolation & purification*
Isoelectric Focusing
Membrane Proteins / isolation & purification*
Ricin / pharmacology*
Reg. No./Substance:
0/Glycoproteins; 0/Membrane Proteins; 9009-86-3/Ricin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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