| Two-dimensional electrophoresis of surface glycoproteins of normal BHK cells and ricin resistant mutants. | |
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MedLine Citation:
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PMID: 760785 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The surface glycoproteins of baby hamster kidney (BHK) cells were iodinated by lactoperoxidase and submitted to a two-dimensional electrophoresis procedure involving isoelectric focusing in the first dimension and SDS gel electrophoresis in the second dimension. After autoradiography a complex but reproducible pattern was obtained. The technique was then applied to the study of three ricin-resistant mutant clones with reduced rates of cell-cell and/or cell-substratum adhesion. Abnormal patterns were observed in all three mutant clones indicating different mechanisms of ricin resistance and identifying glycoproteins which may be involved in cellular interactions. |
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Authors:
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S D Pena; G Mills; R C Hughes |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 550 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1979 Jan |
Date Detail:
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Created Date: 1979-04-25 Completed Date: 1979-04-25 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 100-9 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Adhesion Cell Communication Cell Line Cell Membrane / drug effects, metabolism* Drug Resistance Electrophoresis Glycoproteins / isolation & purification* Isoelectric Focusing Membrane Proteins / isolation & purification* Ricin / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Glycoproteins; 0/Membrane Proteins; 9009-86-3/Ricin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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