Document Detail


Two-dimensional electrophoresis of surface glycoproteins of normal BHK cells and ricin resistant mutants.
MedLine Citation:
PMID:  760785     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The surface glycoproteins of baby hamster kidney (BHK) cells were iodinated by lactoperoxidase and submitted to a two-dimensional electrophoresis procedure involving isoelectric focusing in the first dimension and SDS gel electrophoresis in the second dimension. After autoradiography a complex but reproducible pattern was obtained. The technique was then applied to the study of three ricin-resistant mutant clones with reduced rates of cell-cell and/or cell-substratum adhesion. Abnormal patterns were observed in all three mutant clones indicating different mechanisms of ricin resistance and identifying glycoproteins which may be involved in cellular interactions.
Authors:
S D Pena; G Mills; R C Hughes
Related Documents :
19179445 - Mechanisms of resistance to structurally diverse antiestrogens differ under premenopaus...
15228655 - Activity of telomerase and extracellular regulated protein kinases in parental and drug...
9688315 - Contribution of the cyclin-dependent kinase inhibitor p27kip1 to the confluence-depende...
10101035 - Multiple mechanisms of resistance to polyglutamatable and lipophilic antifolates in mam...
2045925 - Altered reactivity of hemolysate-treated cultured smooth-muscle cells from rabbit basil...
14726685 - Knockdown of chk1, wee1 and myt1 by rna interference abrogates g2 checkpoint and induce...
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  550     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1979 Jan 
Date Detail:
Created Date:  1979-04-25     Completed Date:  1979-04-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  100-9     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Cell Adhesion
Cell Communication
Cell Line
Cell Membrane / drug effects,  metabolism*
Drug Resistance
Electrophoresis
Glycoproteins / isolation & purification*
Isoelectric Focusing
Membrane Proteins / isolation & purification*
Ricin / pharmacology*
Chemical
Reg. No./Substance:
0/Glycoproteins; 0/Membrane Proteins; 9009-86-3/Ricin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Fractionation of membranes from Acholeplasma laidlawii A on the basis of their surface properties by...
Next Document:  Role of unstirred layer in intestinal absorption of phenylalanine in vivo.