| Two-dimensional electrophoresis of human serum proteins following acute myocardial infarction. | |
| | |
MedLine Citation:
|
PMID: 2806207 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Serum proteins associated with acute myocardial infarction (AMI) have been monitored by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and high resolution two-dimensional electrophoresis (2-DE) under nonreducing conditions. Proteins a, b, c (Mr 13,000; pI6.2, 6.7 and 7.5, respectively) and e(Mr27,000; pI5.2) appear simultaneously approximately 30 h after infarction, reach maximum intensity after 48 h and progressively decline thereafter. Protein d (Mr15,000; pI7-8.5; identified as hemoglobin) sometimes appears within 18 h of infarction. Proteins a-c are not detected in the 2-DE patterns of healthy myocardium, infarcted myocardium, pectoral muscle or tongue, but e is present in all and tentatively identified as myosin light chain. Other myocardial proteins which are either reduced in amount following infarction or more specifically associated with myocardium than pectoral muscle are not detected in the serum of AMI patients. Analysis of unconcentrated urine by SDS-PAGE and silver staining does not reveal proteins specific to AMI. |
| | |
Authors:
|
T Marshall; J Williams; K M Williams |
Related Documents
:
|
15242837 - Saturation of high-frequency oscillations of r-r intervals in healthy subjects and pati... 11405397 - Prognostic significance of risk stratifiers of mortality, including t wave alternans, a... 19050597 - Multidecade-long trends (1986-2005) in the utilization of coronary reperfusion and reva... 3776847 - Association between transient pulmonary congestion during acute myocardial infarction a... 16833027 - Cardiovascular disorders and obstructive sleep apnea syndrome. 23432167 - Opium decreases the age at myocardial infarction and sudden cardiac death: a long- and ... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Electrophoresis Volume: 10 ISSN: 0173-0835 ISO Abbreviation: Electrophoresis Publication Date: 1989 Aug-Sep |
Date Detail:
|
Created Date: 1989-12-04 Completed Date: 1989-12-04 Revised Date: 2004-11-17 |
Medline Journal Info:
|
Nlm Unique ID: 8204476 Medline TA: Electrophoresis Country: GERMANY, EAST |
Other Details:
|
Languages: eng Pagination: 584-8 Citation Subset: IM |
Affiliation:
|
Biochemistry Research Laboratory, Department of Biological and Biomedical Sciences, University of Ulster, Coleraine. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adult Aged Aged, 80 and over Blood Proteins / analysis* Electrophoresis, Gel, Two-Dimensional Female Humans Male Middle Aged Myocardial Infarction / blood* Organ Specificity Urine / analysis |
| Chemical | |
Reg. No./Substance:
|
0/Blood Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Heterogeneity of serum creatine kinase isoenzyme-MM in acute myocardial infarction.
Next Document: Diagnostic use of an analysis of urinary proteins by a practicable sodium dodecyl sulfate-electropho...