Document Detail


Two different serum-free media and osmolality effect upon human 293 cell growth and adenovirus production.
MedLine Citation:
PMID:  16314975     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Adenoviruses are promising vectors for gene therapy and vaccination protocols. Consequently, the market demands for adenovirus are increasing, driving the search for new methodologies for large-scale production of concentrated vectors with warranted purity and efficacy, in a cost-effective way. Nevertheless, the production of adenovirus is currently limited by the so-called 'cell density effect', i.e. a drop in cell specific productivity concomitant with increased cell concentration at infection. Of two different serum-free culture media (CD293 and EX-Cell), evaluated for their effect on human 293 cells growth and adenovirus production at cell densities higher than 1x10(6) cells/ml, EX-Cell proved the better medium for cell growth. Although adenovirus production was equivalent in both media when the infection was performed at 1x10(6) cells/ml, at 3x10(6) cells/ml CD293 was the better. This result related to the high ammonia content in EX-Cell medium at the highest cell concentration at infection. Besides this, the large-scale production of these vectors at high cell densities often requires re-feed strategies, which increase medium osmolality. While a negative effect on cell growth was observed with increasing osmolalities, adenovirus productivity was only affected for osmolalities higher than 430 mOsm.
Authors:
Tiago B Ferreira; Ana L Ferreira; Manuel J T Carrondo; Paula M Alves
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Publication Detail:
Type:  Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biotechnology letters     Volume:  27     ISSN:  0141-5492     ISO Abbreviation:  Biotechnol. Lett.     Publication Date:  2005 Nov 
Date Detail:
Created Date:  2005-11-29     Completed Date:  2006-02-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8008051     Medline TA:  Biotechnol Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1809-13     Citation Subset:  IM    
Affiliation:
Instituto de Biologia Experimental e Tecnológica/Instituto de Tecnologia Química e Biológica, Apartado 12, P-2781-901, Oeiras, Portugal.
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MeSH Terms
Descriptor/Qualifier:
Adenoviridae / growth & development*,  isolation & purification*
Cell Culture Techniques / methods*
Cell Line
Cell Proliferation
Culture Media, Serum-Free
Kidney / embryology,  growth & development*,  virology*
Osmolar Concentration
Virus Cultivation / methods*
Chemical
Reg. No./Substance:
0/Culture Media, Serum-Free

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