| Two different serum-free media and osmolality effect upon human 293 cell growth and adenovirus production. | |
| | |
MedLine Citation:
|
PMID: 16314975 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Adenoviruses are promising vectors for gene therapy and vaccination protocols. Consequently, the market demands for adenovirus are increasing, driving the search for new methodologies for large-scale production of concentrated vectors with warranted purity and efficacy, in a cost-effective way. Nevertheless, the production of adenovirus is currently limited by the so-called 'cell density effect', i.e. a drop in cell specific productivity concomitant with increased cell concentration at infection. Of two different serum-free culture media (CD293 and EX-Cell), evaluated for their effect on human 293 cells growth and adenovirus production at cell densities higher than 1x10(6) cells/ml, EX-Cell proved the better medium for cell growth. Although adenovirus production was equivalent in both media when the infection was performed at 1x10(6) cells/ml, at 3x10(6) cells/ml CD293 was the better. This result related to the high ammonia content in EX-Cell medium at the highest cell concentration at infection. Besides this, the large-scale production of these vectors at high cell densities often requires re-feed strategies, which increase medium osmolality. While a negative effect on cell growth was observed with increasing osmolalities, adenovirus productivity was only affected for osmolalities higher than 430 mOsm. |
| | |
Authors:
|
Tiago B Ferreira; Ana L Ferreira; Manuel J T Carrondo; Paula M Alves |
Related Documents
:
|
3494785 - Presence of mitomycin resistant t cells in peripheral blood of normal individuals. 10612605 - Site of photofrin ii photosensitization in larvae of eretmapodites quinquevittatus theo... 6804195 - Flow cytometric characterization of the response of fanconi's anemia cells to mitomycin... 8158795 - Inhibitory effect of minocycline on in vitro invasion and experimental metastasis of mo... 16667135 - Spatial and temporal influences on the cell-specific distribution of glycine decarboxyl... 15744065 - Catfish egg lectin causes rapid activation of multidrug resistance 1 p-glycoprotein as ... |
Publication Detail:
|
Type: Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Biotechnology letters Volume: 27 ISSN: 0141-5492 ISO Abbreviation: Biotechnol. Lett. Publication Date: 2005 Nov |
Date Detail:
|
Created Date: 2005-11-29 Completed Date: 2006-02-28 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 8008051 Medline TA: Biotechnol Lett Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 1809-13 Citation Subset: IM |
Affiliation:
|
Instituto de Biologia Experimental e Tecnológica/Instituto de Tecnologia Química e Biológica, Apartado 12, P-2781-901, Oeiras, Portugal. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adenoviridae
/
growth & development*,
isolation & purification* Cell Culture Techniques / methods* Cell Line Cell Proliferation Culture Media, Serum-Free Kidney / embryology, growth & development*, virology* Osmolar Concentration Virus Cultivation / methods* |
| Chemical | |
Reg. No./Substance:
|
0/Culture Media, Serum-Free |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Biocatalytic amide reduction using Clostridium sporogenes.
Next Document: Isolation of a novel N-acetyl-D-lactosamine specific lectin from Alocasia cucullata (Schott.).