| Two conserved Z9-octadecanoic acid desaturases in the red flour beetle, Tribolium castaneum. | |
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MedLine Citation:
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PMID: 20709164 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Z9 Desaturases catalyse the formation of a cis-unsaturated bond in the Δ9 position of the saturated fatty acids stearate and palmitate. They are considered essential enzymes in eukaryotic organisms as their Z9 unsaturated fatty acid products are required for homeostatic roles such as maintenance of membrane fluidity. Two putative Z9 acyl Coenzyme-A desaturase genes were identified in the red flour beetle, Tribolium castaneum, genome (TcasZ9desA and B) based on their similarity to acyl CoA-desaturases of other insects. TcasZ9desA and B share 75% nucleic acid sequence identity and appear to be functionally conserved; the genes were cloned and expressed in the yeast strain Saccharomyces cerevisiae (ole1); both genes complemented the yeast requirement for Z9 fatty acids and produced substantial quantities of Z9 desaturated products with a stearate>palmitate chain length preference. Quantitative PCR analysis of transcripts in RNA obtained from adult, larval and pupal stages of the beetles show TcasZ9desA and B are expressed at similar levels in all stages, with the pupal stage having the lowest expression. |
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Authors:
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Irene Horne; Nerida Gibb; Katherine Damcevski; Karen Glover; Victoria S Haritos |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-08-13 |
Journal Detail:
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Title: Gene Volume: 468 ISSN: 1879-0038 ISO Abbreviation: Gene Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-09-27 Completed Date: 2010-10-12 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7706761 Medline TA: Gene Country: Netherlands |
Other Details:
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Languages: eng Pagination: 41-7 Citation Subset: IM |
Copyright Information:
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Crown Copyright © 2010. Published by Elsevier B.V. All rights reserved. |
Affiliation:
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CSIRO Entomology, GPO Box 1700, Canberra, ACT 2601, Australia. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Chromatography, Gas Conserved Sequence / genetics* Esters / analysis Evolution, Molecular Gene Expression Regulation, Developmental Gene Expression Regulation, Enzymologic Genome, Insect / genetics Mixed Function Oxygenases / chemistry, genetics* Molecular Sequence Data Phylogeny Sequence Alignment Sequence Analysis, DNA Sequence Homology, Amino Acid Tribolium / enzymology*, genetics*, growth & development |
| Chemical | |
Reg. No./Substance:
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0/Esters; EC 1.-/Mixed Function Oxygenases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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