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Two-component system GacS/A activates lipA translation by RsmE but not RsmA in Pseudomonas protegens Pf-5.
MedLine Citation:
PMID:  25128345     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
In Pseudomonas spp., the Gac/Rsm signal transduction system is required for the production of lipases. The current model assumes that the system induces lipase gene transcription mediated through the quorum sensing (QS) system. However, there are no reports of a QS system based upon N-acyl homoserine lactones or regulation of lipase gene expression in P. protegens. In this study, we investigated the regulatory mechanism acting on lipA expression activated by the Gac/Rsm system in P. protegens Pf-5 through deletion and overexpression of gacA, overexpression of rsmA or rsmE, expression of various lacZ fusions, RT-PCR analysis, and determination of whole-cell lipase activity. The results demonstrated that the GacS/A system activated lipA expression at both the transcriptional and translational levels but that the translational level was the key regulatory pathway. Further results showed that the activation of lipA translation by the GacS/A system was mediated through RsmE, which inhibited lipA translation by binding to the ACAAGGAUGU sequence overlapping the SD sequence of lipA mRNA to hinder the access of the 30S ribosomal subunit to the SD sequence. Moreover, the GacS/A system promoted lipA transcription through the mediation of RsmA inhibiting lipA transcription via an unknown pathway. Besides the transcriptional repression, RsmA mainly activated lipA translation by negatively regulating rsmE translation. In summary, in P. protegens Pf-5 the Gac/RsmE system mainly and directly activates lipA translation and the Gac/RsmA system indirectly enhances lipA transcription.
Authors:
Daiming Zha; Li Xu; Houjin Zhang; Yunjun Yan
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-8-15
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  -     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2014 Aug 
Date Detail:
Created Date:  2014-8-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2014, American Society for Microbiology. All Rights Reserved.
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