| Two cell-cycle regulated SET-domain proteins interact with proliferating cell nuclear antigen (PCNA) in Arabidopsis. | |
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MedLine Citation:
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PMID: 16771839 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The proliferating cell nuclear antigen (PCNA) functions as a sliding clamp for DNA polymerase, and is thus a key actor in DNA replication. It is also involved in DNA repair, maintenance of heterochromatic regions throughout replication, cell cycle regulation and programmed cell death. Identification of PCNA partners is therefore necessary for understanding these processes. Here we identify two Arabidopsis SET-domain proteins that interact with PCNA: ATXR5 and ATXR6. A truncated ATXR5Deltaex2, incapable of interacting with PCNA, also occurs in planta. ATXR6, upregulated during the S phase, is upregulated by AtE2F transcription factors, suggesting that it is required for S-phase progression. The two proteins differ in their subcellular localization: ATXR5 has a dual localization in plastids and in the nucleus, whereas ATXR6 is solely nuclear. This indicates that the two proteins may play different roles in plant cells. However, overexpression of either ATXR5 or ATXR6 causes male sterility because of the degeneration of defined cell types. Taken together, our results suggest that both proteins may play a role in the cell cycle or DNA replication, and that the activity of ATXR5 may be regulated via its subcellular localization. |
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Authors:
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Cécile Raynaud; Rosangela Sozzani; Nathalie Glab; Séverine Domenichini; Claudette Perennes; Rino Cella; Eva Kondorosi; Catherine Bergounioux |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-06-12 |
Journal Detail:
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Title: The Plant journal : for cell and molecular biology Volume: 47 ISSN: 0960-7412 ISO Abbreviation: Plant J. Publication Date: 2006 Aug |
Date Detail:
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Created Date: 2006-08-21 Completed Date: 2006-10-06 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9207397 Medline TA: Plant J Country: England |
Other Details:
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Languages: eng Pagination: 395-407 Citation Subset: IM |
Affiliation:
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Institut de Biotechnologies des Plantes, CNRS UMR 8618, Bâtiment 630, Université Paris-Sud XI, 91405 Orsay, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Motifs Arabidopsis / cytology, genetics, metabolism* Arabidopsis Proteins / chemistry, genetics, metabolism* Cell Cycle / physiology* Cell Cycle Proteins / chemistry, genetics, metabolism* Cell Nucleus / metabolism Cell Proliferation DNA Replication E2F Transcription Factors / metabolism, physiology Flowers / growth & development, metabolism Gene Expression Regulation, Plant Green Fluorescent Proteins / analysis Nuclear Proteins / chemistry, genetics, metabolism* Plant Infertility Plastids / metabolism Proliferating Cell Nuclear Antigen / metabolism* Protein Structure, Tertiary Recombinant Fusion Proteins / analysis Two-Hybrid System Techniques |
| Chemical | |
Reg. No./Substance:
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0/Arabidopsis Proteins; 0/Cell Cycle Proteins; 0/E2F Transcription Factors; 0/Nuclear Proteins; 0/Proliferating Cell Nuclear Antigen; 0/Recombinant Fusion Proteins; 0/XR5 protein, Arabidopsis; 147336-22-9/Green Fluorescent Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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