Document Detail


Two cassava promoters related to vascular expression and storage root formation.
MedLine Citation:
PMID:  13680228     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cassava ( Manihot esculenta Crantz) storage roots, organs accumulating large amounts of starch, develop from primary roots via secondary growth. The availability of promoters related to storage-root formation is a prerequisite for engineering root traits in cassava. Two cDNAs, c15 and c54, were identified from a storage-root cDNA library of cassava MCol1505 via differential screening. The transcripts of c15 and c54 were detected in storage roots but not in leaves by Northern analysis. Homology analysis of the deduced amino acid sequences showed that C15 is likely to be related to cytochrome P450 proteins, which are involved in the oxidative degradation of various compounds, while C54 may be related to Pt2L4, a cassava glutamic acid-rich protein. The promoter regions of c15 and c54 were isolated from the corresponding clones in a cassava genomic library. A 1,465-bp promoter fragment ( p15/1.5) of c15 and a 1,081-bp promoter region ( p54/1.0) of c54 were translationally fused to the uidA reporter gene, and introduced into cassava and Arabidopsis thaliana (L.) Heynh. The expression patterns of p15/1.5::uidA and p54/1.0::uidA in transgenic plants showed that both promoters are predominantly active in phloem, cambium and xylem vessels of vascular tissues from leaves, stems, and root systems. More importantly, strong beta-glucuronidase activity was also detected in the starch-rich parenchyma cells of transgenic storage roots. Our results demonstrate that the two promoters are related to vascular expression and secondary growth of storage roots in cassava.
Authors:
Peng Zhang; Susanne Bohl-Zenger; Johanna Puonti-Kaerlas; Ingo Potrykus; Wilhelm Gruissem
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2003-09-10
Journal Detail:
Title:  Planta     Volume:  218     ISSN:  0032-0935     ISO Abbreviation:  Planta     Publication Date:  2003 Dec 
Date Detail:
Created Date:  2003-12-03     Completed Date:  2004-03-16     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  1250576     Medline TA:  Planta     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  192-203     Citation Subset:  IM    
Affiliation:
Institute of Plant Sciences, ETH-Zentrum / LFW E 17, Universitätstrasse 2, 8092 Zürich, Switzerland. zhang.peng@ipw.biol.ethz.ch
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AY217351;  AY217352;  AY217353;  AY217354
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Base Sequence
Biological Transport / genetics
Cloning, Molecular
Cytochrome P-450 Enzyme System / genetics,  metabolism
DNA, Complementary / chemistry,  genetics
Gene Expression Regulation, Developmental
Gene Expression Regulation, Plant
Glucuronidase / genetics,  metabolism
Manihot / genetics*,  growth & development,  metabolism
Molecular Sequence Data
Plant Proteins / genetics*,  metabolism
Plant Roots / genetics*,  growth & development,  metabolism
Plants, Genetically Modified
Promoter Regions, Genetic / genetics*
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Chemical
Reg. No./Substance:
0/DNA, Complementary; 0/Plant Proteins; 9035-51-2/Cytochrome P-450 Enzyme System; EC 3.2.1.31/Glucuronidase

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