Document Detail


Two aerobic pathways for the formation of unsaturated fatty acids in Pseudomonas aeruginosa.
MedLine Citation:
PMID:  16573679     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The double bond in anaerobic unsaturated fatty acid (UFA) biosynthesis is introduced by the FabA dehydratase/isomerase of the bacterial type II fatty acid biosynthetic pathway. A DeltafabA mutant of Pseudomonas aeruginosa grew aerobically, but required a UFA supplement for anaerobic growth. Wild-type cells produced 18:1Delta11 as the principal UFA, whereas the DeltafabA strain produced only 16:1Delta9. The double bond in the 16:1Delta9 was introduced after phospholipid formation and was localized in the sn-2 position. Two predicted membrane proteins, DesA and DesB, possessed the conserved histidine clusters characteristic of fatty acid desaturases. The DeltafabADeltadesA double mutant required exogenous fatty acids for growth but the DeltafabAdesB double mutant did not. Exogenous stearate was converted to 18:1Delta9 and supported the growth of DeltafabADeltadesA double mutant. A DeltafabADeltadesAdesB triple mutant was unable to desaturate exogenous stearate and was an UFA auxotroph. We detected a 2.5-fold increase in desA expression in DeltafabA mutants, whereas desB expression was derepressed by the deletion of the gene encoding a transcriptional repressor DesT. These data add two aerobic desaturases to the enzymes used for fatty acid metabolism in proteobacteria: DesA, a 2-position phospholipid Delta9-desaturase that supplements the anaerobic FabA pathway, and DesB, an inducible acyl-CoA Delta9-desaturase whose expression is repressed by DesT.
Authors:
Kun Zhu; Kyoung-Hee Choi; Herbert P Schweizer; Charles O Rock; Yong-Mei Zhang
Related Documents :
22092299 - Thiamine plays a critical role in the acid tolerance of listeria monocytogenes.
9934539 - Val 70, phe 72 and the last seven amino acid residues of c-terminal are essential to th...
18832479 - Key amino acid residues responsible for the differences in substrate specificity of hum...
2636039 - The structures of the lipopolysaccharide core components from rhizobium leguminosarum b...
16927319 - Determination of the cryptic stereochemistry of the first pks chain-extension step in a...
10923709 - Inoculation and nitrate alter phytohormone levels in soybean roots: differences between...
429099 - Isolation and characterization of fin whale prolactin.
19130569 - A novel, environmentally friendly sodium lauryl ether sulfate-, cocamidopropyl betaine-...
9866169 - Oxidized resin acids in aerosol derived from rosin core solder.
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular microbiology     Volume:  60     ISSN:  0950-382X     ISO Abbreviation:  Mol. Microbiol.     Publication Date:  2006 Apr 
Date Detail:
Created Date:  2006-03-31     Completed Date:  2006-06-27     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8712028     Medline TA:  Mol Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  260-73     Citation Subset:  IM    
Affiliation:
Department of Infectious Diseases, Protein Science Division, St Jude Children's Research Hospital, Memphis, TN 38105, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Aerobiosis / genetics
Amino Acid Sequence
Cell Membrane / enzymology
Fatty Acids, Unsaturated / biosynthesis*,  genetics
Gene Deletion
Gene Expression Regulation, Bacterial
Genes, Bacterial
Hydro-Lyases / genetics
Membrane Proteins / genetics,  metabolism*
Molecular Sequence Data
Multigene Family
Pseudomonas aeruginosa / enzymology*,  genetics
Stearates / metabolism
Stearoyl-CoA Desaturase / genetics,  metabolism*
Substrate Specificity
Grant Support
ID/Acronym/Agency:
CA21765/CA/NCI NIH HHS; GM34496/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Fatty Acids, Unsaturated; 0/Membrane Proteins; 0/Stearates; EC 1.14.19.1/Stearoyl-CoA Desaturase; EC 4.2.1.-/Hydro-Lyases
Comments/Corrections
Comment In:
Mol Microbiol. 2006 Apr;60(2):256-9   [PMID:  16573678 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  What is life? The paradigm of DNA and protein cooperation at high local concentrations.
Next Document:  Characterizing the flagellar filament and the role of motility in bacterial prey-penetration by Bdel...