Document Detail


Two zinc uptake systems contribute to the full virulence of Listeria monocytogenes during growth in vitro and in vivo.
MedLine Citation:
PMID:  22025520     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We report here the identification and characterization of two zinc uptake systems, ZurAM and ZinABC, in the intracellular pathogen Listeria monocytogenes. Transcription of both operons was zinc responsive and regulated by the zinc-sensing repressor Zur. Deletion of either zurAM or zinA had no detectable effect on growth in defined media, but a double zurAM zinA mutant was unable to grow in the absence of zinc supplementation. Deletion of zinA had no detectable effect on intracellular growth in HeLa epithelial cells. In contrast, growth of the zurAM mutant was significantly impaired in these cells, indicating the importance of the ZurAM system during intracellular growth. Notably, the deletion of both zinA and zurAM severely attenuated intracellular growth, with the double mutant being defective in actin-based motility and unable to spread from cell to cell. Deletion of either zurAM or zinA had a significant effect on virulence in an oral mouse model, indicating that both zinc uptake systems are important in vivo and establishing the importance of zinc acquisition during infection by L. monocytogenes. The presence of two zinc uptake systems may offer a mechanism by which L. monocytogenes can respond to zinc deficiency within a variety of environments and during different stages of infection, with each system making distinct contributions under different stress conditions.
Authors:
David Corbett; Jiahui Wang; Stephanie Schuler; Gloria Lopez-Castejon; Sarah Glenn; David Brough; Peter W Andrew; Jennifer S Cavet; Ian S Roberts
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-10-24
Journal Detail:
Title:  Infection and immunity     Volume:  80     ISSN:  1098-5522     ISO Abbreviation:  Infect. Immun.     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2011-12-26     Completed Date:  2012-02-16     Revised Date:  2014-02-20    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  14-21     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Transport
Colony Count, Microbial
Cytoplasm / microbiology
Disease Models, Animal
Gene Expression Profiling
Gene Expression Regulation, Bacterial / drug effects
HeLa Cells
Humans
Listeria monocytogenes / genetics,  metabolism*,  pathogenicity*
Listeriosis / microbiology,  mortality,  pathology
Membrane Transport Proteins / genetics,  metabolism*
Mice
Operon
Survival Analysis
Transcription, Genetic
Virulence
Zinc / metabolism*
Grant Support
ID/Acronym/Agency:
G0601205//Medical Research Council; //Medical Research Council
Chemical
Reg. No./Substance:
0/Membrane Transport Proteins; J41CSQ7QDS/Zinc
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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